5.4.99.2: methylmalonyl-CoA mutase
This is an abbreviated version!
For detailed information about methylmalonyl-CoA mutase, go to the full flat file.
Word Map on EC 5.4.99.2
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5.4.99.2
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methylmalonic
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acidemia
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acidurias
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adenosylcobalamin
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propionate
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succinyl-coa
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inborn
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b12-dependent
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propionyl-coa
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adocbl
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homocysteine
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methylcobalamin
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apoenzyme
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shermanii
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adenosylcobalamin-dependent
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5\'-deoxyadenosylcobalamin
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propionibacterium
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hyperammonemia
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homolysis
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adenosylation
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homocystinuria
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mutases
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propionylcarnitine
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adenosyltransferase
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mmaa
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transcobalamins
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methylmalonyl-coenzyme
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propionyl
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adocbl-dependent
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odd-chain
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megaloblastic
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cobalt-carbon
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cyanocobalamin
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mmachc
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cobiialamin
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b12-binding
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hydroxycobalamin
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medicine
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mecbl
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cobalamin-binding
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cinnamonensis
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5\'-deoxyadenosyl
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isobutyryl-coa
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analysis
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extorquens
- 5.4.99.2
-
methylmalonic
- acidemia
- acidurias
- adenosylcobalamin
- propionate
- succinyl-coa
-
inborn
-
b12-dependent
- propionyl-coa
-
adocbl
- homocysteine
- methylcobalamin
-
apoenzyme
- shermanii
-
adenosylcobalamin-dependent
-
5\'-deoxyadenosylcobalamin
- propionibacterium
-
hyperammonemia
-
homolysis
-
adenosylation
- homocystinuria
- mutases
- propionylcarnitine
-
adenosyltransferase
- mmaa
-
transcobalamins
-
methylmalonyl-coenzyme
-
propionyl
-
adocbl-dependent
-
odd-chain
-
megaloblastic
-
cobalt-carbon
- cyanocobalamin
- mmachc
-
cobiialamin
-
b12-binding
- hydroxycobalamin
- medicine
-
mecbl
-
cobalamin-binding
- cinnamonensis
-
5\'-deoxyadenosyl
- isobutyryl-coa
- analysis
- extorquens
Reaction
Synonyms
(R)-2-methyl-3-oxopropanoyl-CoA CoA-carbonylmutase, (S)-Methylmalonyl-CoA mutase, cobalamin-dependent methylmalonyl-CoA mutase, hMCM, L-methylmalonyl-co-enzyme-A mutase, L-methylmalonyl-CoA mutase, MCB-beta, MCM, MCM-alpha, MCM-beta, mcmB, Methylmalonyl CoA mutase, Methylmalonyl coenzyme A carbonylmutase, Methylmalonyl coenzyme A mutase, methylmalonyl-CoA mutase, Methylmalonyl-CoA-carbonyl mutase, mitochondrial methylmalonyl-CoA mutase, mmcm-1, Msed_0638, Msed_2055, MuT, Mutase, methylmalonyl coenzyme A, Sbm, sleeping beauty mutase
ECTree
5.4.99.2 methylmalonyl-CoA mutaseAdvanced search results
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results (Purification
Purification on EC 5.4.99.2 - methylmalonyl-CoA mutase
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PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, TSKgel phenyl-Toyopearl column chromatography, TSKgel QAE-Toyopearl column chromatography, Bio-Scale CHT2-1 column chromatography, UNO Q-1 column chromatography, and Superdex 200 gel filtration
Ni-NTA column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta(DE3)pLysS cells by nickel affinity and anion exchange chromatography
recombinant His6-tagged wild-type and N-terminal deletion mutant of PH1306 from Escherichia coli strain BL21 Star (DE3) pLysS inclusion bodies by heat treatment at 95°C for 5 min, nickel affinity chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, anion exchange chromatography, and gel filtration. rPH0275 is produced in the soluble fraction with high efficiency and purified to homogeneity by nickel affinity chromatography. To obtain substantial quantities of soluble rPH1306, the soluble mutant rPH1306DELTAN is used
O74009; O58013
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