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Literature summary for 5.4.99.2 extracted from

  • Takahashi-Iniguez, T.; Garcia-Arellano, H.; Trujillo-Roldan, M.A.; Flores, M.E.
    Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein (2011), Biochem. Biophys. Res. Commun., 404, 443-447.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
methylmalonic acidemia protein after 60 min of reaction, when MCM is inactive, the addition of methylmalonic acidemia protein increases the enzymatic activity through GTP hydrolysis, indicating reactivation of MCM by exchange of the damaged cofactor. Methylmalonic acidemia protein acts as a chaperone of human MCM Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
IMAC column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-methylmalonyl-CoA
-
Homo sapiens succinyl-CoA
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
MCM
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
adenosylcobalamin MCM activity with adenosylcobalamin increases linearly during the first 3-4 min Homo sapiens