Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methylmalonic acidemia protein | after 60 min of reaction, when MCM is inactive, the addition of methylmalonic acidemia protein increases the enzymatic activity through GTP hydrolysis, indicating reactivation of MCM by exchange of the damaged cofactor. Methylmalonic acidemia protein acts as a chaperone of human MCM | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
IMAC column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-methylmalonyl-CoA | - |
Homo sapiens | succinyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
MCM | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
adenosylcobalamin | MCM activity with adenosylcobalamin increases linearly during the first 3-4 min | Homo sapiens |