5.4.99.14: 4-carboxymethyl-4-methylbutenolide mutase

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For detailed information about 4-carboxymethyl-4-methylbutenolide mutase, go to the full flat file.

Reaction

4-carboxymethyl-4-methylbut-2-en-1,4-olide

4-Carboxymethyl-3-methylbut-2-en-1,4-olide

Synonyms

4-Methyl-3-enelactone methyl isomerase, 4-Methylmuconolactone methylisomerase, Isomerase, 4-methyl-2-enelactone, methymuconolactone isomerase, MLMI, MmlJ

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.99 Transferring other groups

5.4.99.14 4-carboxymethyl-4-methylbutenolide mutase

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Results	in table
2	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
1	General Stability
26	Inhibitors
4	kcat/KM [mM/s]
8	KM Value [mM]
2	Localization
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
12	PDB ID
3	pH Optimum
1	pH Range
4	Protein Variants
4	Purification (Commentary)
4	Reaction
1	Reaction Type
14	Reference
9	Source Tissue
4	Specific Activity [micromol/min/mg]
3	Storage Stability
27	Substrates and Products (Substrate)
6	Subunits
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
6	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 5.4.99.14 - 4-carboxymethyl-4-methylbutenolide mutase

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12900

Cupriavidus necator

O51799

2 * 12900, about, sequence calculation

717634

17000

Rhodococcus rhodochrous

4 * 17000, Rhodococcus rhodochrous, SDS-PAGE

25000

gel filtration

40000

1 * 40000, Alcaligenes eutrophus (JMP 134, cloned into Pseudomonas sp. B13 FR1), SDS-PAGE

3483

75500

Rhodococcus rhodochrous

Rhodococcus rhodochrous, gel filtration

3482