5.4.99.14: 4-carboxymethyl-4-methylbutenolide mutase

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For detailed information about 4-carboxymethyl-4-methylbutenolide mutase, go to the full flat file.

Reaction

4-carboxymethyl-4-methylbut-2-en-1,4-olide

4-Carboxymethyl-3-methylbut-2-en-1,4-olide

Synonyms

4-Methyl-3-enelactone methyl isomerase, 4-Methylmuconolactone methylisomerase, Isomerase, 4-methyl-2-enelactone, methymuconolactone isomerase, MLMI, MmlJ

ECTree

5 Isomerases

5.4 Intramolecular transferases

5.4.99 Transferring other groups

5.4.99.14 4-carboxymethyl-4-methylbutenolide mutase

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Results	in table
2	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
1	General Stability
26	Inhibitors
4	kcat/KM [mM/s]
8	KM Value [mM]
2	Localization
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
12	PDB ID
3	pH Optimum
1	pH Range
4	Protein Variants
4	Purification (Commentary)
4	Reaction
1	Reaction Type
14	Reference
9	Source Tissue
4	Specific Activity [micromol/min/mg]
3	Storage Stability
27	Substrates and Products (Substrate)
6	Subunits
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
6	Turnover Number [1/s]

Crystallization

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Crystallization on EC 5.4.99.14 - 4-carboxymethyl-4-methylbutenolide mutase

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in complex with (4S)-4-methylmuconolactone and (4S)-3-methylmuconolactone, sitting drop vapor diffusion method, at 19°C, using 25% (w/v) polyethylene glycol 1500, 0.1 M MMT buffer, pH 5 (DL-malic acid, MES, and Tris base in a 1:2:2 molar ratio)

Pseudomonas reinekei

C5MR76

704578