5.4.99.14: 4-carboxymethyl-4-methylbutenolide mutase

This is an abbreviated version!
For detailed information about 4-carboxymethyl-4-methylbutenolide mutase, go to the full flat file.

Reaction

Synonyms

4-Methyl-3-enelactone methyl isomerase, 4-Methylmuconolactone methylisomerase, Isomerase, 4-methyl-2-enelactone, methymuconolactone isomerase, MLMI, MmlJ

ECTree

     5 Isomerases

         5.4 Intramolecular transferases

             5.4.99 Transferring other groups

                5.4.99.14 4-carboxymethyl-4-methylbutenolide mutase

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Results	in table
2	AA Sequence
1	CAS Registry Number
3	Cloned(Commentary)
1	Crystallization (Commentary)
2	General Information
1	General Stability
26	Inhibitors
4	kcat/KM [mM/s]
8	KM Value [mM]
2	Localization
5	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
18	Organism
3	Pathway
12	PDB ID
3	pH Optimum
1	pH Range
4	Protein Variants
4	Purification (Commentary)
4	Reaction
1	Reaction Type
14	Reference
9	Source Tissue
4	Specific Activity [micromol/min/mg]
3	Storage Stability
27	Substrates and Products (Substrate)
6	Subunits
12	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
6	Turnover Number [1/s]

Engineering

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Engineering on EC 5.4.99.14 - 4-carboxymethyl-4-methylbutenolide mutase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

C67S

Pseudomonas reinekei

C5MR76

incubation of variant C67S with 4-chloromercuribenzoate does not significantly decrease its activity (above 65% of activity remaining after a 30-min incubation)

704578

H26A

Pseudomonas reinekei

C5MR76

the mutant shows a marked decrease in catalytic activity (kcat) by two orders of magnitude

704578

H52A

Pseudomonas reinekei

C5MR76

the mutation results in an increase in Km by a factor of 9, but only a small change in kcat

704578

Y39F

Pseudomonas reinekei

C5MR76

the mutant displays reduced activity compared to the wild type enzyme

704578