Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(+)-muconolactone | - |
Rhodococcus rhodochrous | |
(-)1-Methyl-8-bromobislactone | weak | Rhodococcus rhodochrous | |
2-Bromo-4-methylbutan-1,4-olide | weak | Rhodococcus rhodochrous | |
2-Bromobutan-1,4-olide | - |
Rhodococcus rhodochrous | |
2-Methylbutan-1,4-olide | - |
Rhodococcus rhodochrous | |
4-Methylbut-3-en-1,4-olide | angelicalactone | Rhodococcus rhodochrous | |
4-methylbutan-1,4-olide | - |
Rhodococcus rhodochrous | |
AgNO3 | - |
Rhodococcus rhodochrous | |
CuSO4 | - |
Rhodococcus rhodochrous | |
HgCl2 | - |
Rhodococcus rhodochrous | |
additional information | EDTA | Rhodococcus rhodochrous | |
PCMB | - |
Rhodococcus rhodochrous |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
1-methyl-bislactone | - |
Rhodococcus rhodochrous | |
0.23 | - |
4-carboxymethyl-4-methylbut-2-en-1,4-olide | - |
Rhodococcus rhodochrous |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rhodococcus rhodochrous | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
4 * 17000, Rhodococcus rhodochrous, SDS-PAGE | Rhodococcus rhodochrous |
75500 | - |
Rhodococcus rhodochrous, gel filtration | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
N75 | - |
Rhodococcus rhodochrous N75 | - |
N75 | - |
Purification (Comment) | Organism |
---|---|
N75 | Rhodococcus rhodochrous |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
4-Carboxymethyl-4-methylbut-2-en-1,4-olide = 4-carboxymethyl-3-methylbut-2-en-1,4-olide | isomerization does not involve actual methyl migration, but proceeds via the 1-methylbislactone | Rhodococcus rhodochrous |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:p-toluate-grown cell | - |
Rhodococcus rhodochrous | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
785 | - |
- |
Rhodococcus rhodochrous |
Storage Stability | Organism |
---|---|
-30°C, slow loss of activity on prolonged storage | Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-Methylbislactone | - |
Rhodococcus rhodochrous | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | - |
? | |
1-Methylbislactone | - |
Rhodococcus rhodochrous N75 | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | - |
? | |
4-Carboxymethyl-4-methylbut-2-en-1,4-olide | highly specific, accepts only (+)-(4S)-4-methylmuconolactone, i.e. 4-carboxymethyl-4-methylbut-2-en-1,4-olide and the putative reaction intermediate 1-methylbislactone | Rhodococcus rhodochrous | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | i.e. (-)-(4S)-3-methylmuconolactone | ? | |
4-Carboxymethyl-4-methylbut-2-en-1,4-olide | highly specific, accepts only (+)-(4S)-4-methylmuconolactone, i.e. 4-carboxymethyl-4-methylbut-2-en-1,4-olide and the putative reaction intermediate 1-methylbislactone | Rhodococcus rhodochrous N75 | 4-Carboxymethyl-3-methylbut-2-en-1,4-olide | i.e. (-)-(4S)-3-methylmuconolactone | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 17000, Rhodococcus rhodochrous, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus rhodochrous |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | active in this range, rapid decrease of activity above pH 8.5 | Rhodococcus rhodochrous |