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5.4.99.1: methylaspartate mutase

This is an abbreviated version!
For detailed information about methylaspartate mutase, go to the full flat file.

Word Map on EC 5.4.99.1

Reaction

L-threo-3-methylaspartate
=
L-glutamate

Synonyms

AdoCbl-dependent glutamate mutase, Glm, GlmS, Glutamate isomerase, Glutamate mutase, Glutamic acid isomerase, Glutamic acid mutase, Glutamic isomerase, Glutamic mutase, Methylaspartic acid mutase, Mutase, methylaspartate, mutE, mutS, SanU, SanV

ECTree

     5 Isomerases
         5.4 Intramolecular transferases
             5.4.99 Transferring other groups
                5.4.99.1 methylaspartate mutase

Cloned

Cloned on EC 5.4.99.1 - methylaspartate mutase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
13C,15N-labeled MutS, the coenzyme B12-binding subunit of glutamate mutase prepared by overexpression from Escherichia coli
-
expressed in Escherichia coli
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expression in Escherichia coli
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genes mutS and mutE, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) pLysS
glutamate mutase S component MutS. Induction strategy to enhance the level of protein expression in bacteriophage T7 expression system in Escherichia coli. Yield of purified protein is increased threefold by reducing the induction temperature to 20°C and using 0.2% lactose and 50 mg per l IPTG simultaneously as inducers
-
individual expression of SanU and SanV in Escherichia coli
-
overexpression of component E and S in Escherichia coli
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overexpression of polypeptide chains sigma and epsilon in Escherichia coli
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polypeptide chains sigma and epsilon expressed in Escherichia coli