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Literature summary for 5.4.99.1 extracted from

  • Holloway, D.E.; Marsh, E.N.G.
    Adenosylcobalamin-dependent glutamate mutase from Clostridium tetanomorphum. Overexpression in Escherichia coli, purification, and characterization of the recombinant enzyme (1994), J. Biol. Chem., 269, 20425-20430.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of component E and S in Escherichia coli Clostridium tetanomorphum

Organism

Organism UniProt Comment Textmining
Clostridium tetanomorphum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cloning and overexpression in Escherichia coli allows component E to be obtained in homogeneous form, free of inhibiting cobamides and traces of component S Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
component E Clostridium tetanomorphum

Storage Stability

Storage Stability Organism
-20°C, component E in presence of 50% glycerol, stable for several months Clostridium tetanomorphum
4°C or -20°C, component S in concentrated solution in the absence of thiols, stable for several months Clostridium tetanomorphum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Glu
-
Clostridium tetanomorphum threo-3-Methylaspartate
-
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