Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.4.99.1 extracted from

  • Li, Y.; Ling, H.; Li, W.; Tan, H.
    Improvement of nikkomycin production by enhanced copy of sanU and sanV in Streptomyces ansochromogenes and characterization of a novel glutamate mutase encoded by sanU and sanV (2005), Metab. Eng., 7, 165-173.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
individual expression of SanU and SanV in Escherichia coli Streptomyces ansochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
L-threo-3-methylaspartate 37°C Streptomyces ansochromogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.1 mol per mol of subunit SanU Streptomyces ansochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV Streptomyces ansochromogenes
18370
-
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV Streptomyces ansochromogenes
47850
-
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV Streptomyces ansochromogenes
48000
-
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV Streptomyces ansochromogenes

Organism

Organism UniProt Comment Textmining
Streptomyces ansochromogenes
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information sulfhydryl compounds are not essential for maintaining active SanU, but for activity of reassembled enzyme compolex Streptomyces ansochromogenes

Purification (Commentary)

Purification (Comment) Organism
after individual expression of SanU and SanV in Escherichia coli Streptomyces ansochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Streptomyces ansochromogenes L-threo-3-methylaspartate
-
?

Subunits

Subunits Comment Organism
? x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV Streptomyces ansochromogenes
More no enzymic activity for subunit SanU or SanV alone, enzymic activity results after mixing both subunits plus coenzyme B12, reversible and dynamical equilibrium between SanU and SanV in formation of a possibly tetrameric complex Streptomyces ansochromogenes

Synonyms

Synonyms Comment Organism
SanU
-
Streptomyces ansochromogenes
SanV
-
Streptomyces ansochromogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 42
-
Streptomyces ansochromogenes

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Streptomyces ansochromogenes

Cofactor

Cofactor Comment Organism Structure
coenzyme B12 binds to subunit SanU with about 0.1 mol Co per mol SanU Streptomyces ansochromogenes