Cloned (Comment) | Organism |
---|---|
individual expression of SanU and SanV in Escherichia coli | Streptomyces ansochromogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
L-threo-3-methylaspartate | 37°C | Streptomyces ansochromogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.1 mol per mol of subunit SanU | Streptomyces ansochromogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
18370 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
47850 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
48000 | - |
x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces ansochromogenes | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | sulfhydryl compounds are not essential for maintaining active SanU, but for activity of reassembled enzyme compolex | Streptomyces ansochromogenes |
Purification (Comment) | Organism |
---|---|
after individual expression of SanU and SanV in Escherichia coli | Streptomyces ansochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Streptomyces ansochromogenes | L-threo-3-methylaspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 18000, SDS-PAGE, x * 18370, calcultated, subunit SanU, x * 48000, SDS-PAGE, x * 47850, calculated, subunit SanV | Streptomyces ansochromogenes |
More | no enzymic activity for subunit SanU or SanV alone, enzymic activity results after mixing both subunits plus coenzyme B12, reversible and dynamical equilibrium between SanU and SanV in formation of a possibly tetrameric complex | Streptomyces ansochromogenes |
Synonyms | Comment | Organism |
---|---|---|
SanU | - |
Streptomyces ansochromogenes |
SanV | - |
Streptomyces ansochromogenes |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 42 | - |
Streptomyces ansochromogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Streptomyces ansochromogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
coenzyme B12 | binds to subunit SanU with about 0.1 mol Co per mol SanU | Streptomyces ansochromogenes |