5.1.1.21: isoleucine 2-epimerase

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For detailed information about isoleucine 2-epimerase, go to the full flat file.

Reaction

Synonyms

amino acid racemase, BCAA racemase, branched-chain amino-acid racemase, D-amino acid racemase, DAAR1, ILEP, isoleucine 2-epimerase, phenazine biosynthesis PhzC/PhzF family protein, PLP-dependent fold-type I isoleucine 2-epimerase, PLP-independent racemase

ECTree

     5 Isomerases

         5.1 Racemases and epimerases

             5.1.1 Acting on amino acids and derivatives

                5.1.1.21 isoleucine 2-epimerase

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Results	in table
37	AA Sequence
4	Cloned(Commentary)
5	Cofactor
2	Crystallization (Commentary)
10	General Information
2	Inhibitors
11	kcat/KM [mM/s]
11	KM Value [mM]
3	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
10	Organism
16	PDB ID
3	pH Optimum
1	pH Stability
4	Protein Variants
5	Purification (Commentary)
4	Reaction
8	Reference
1	Source Tissue
17	Specific Activity [micromol/min/mg]
1	Storage Stability
35	Substrates and Products (Substrate)
4	Subunits
17	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
11	Turnover Number [1/s]

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Cloned on EC 5.1.1.21 - isoleucine 2-epimerase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli

Lentilactobacillus buchneri

M1GRN3

729935

gene daar1, genotyping, untargeted metabolite profiling and genome-wide association analysis on 440 natural accessions of Arabidopsis thaliana to establish genetic linkages between metabolites and genes, recombinant expression of His-tagged enzyme DAAR1 in Escherichia coli strain Rosetta 2 pLysS. The affinity tag does not affect enzyme activity or stability

Arabidopsis thaliana

Q66GM9

749170

recombinant expression of codon-optimized, His-tagged enzyme in Escherichia coli strain BL21(DE3)

Lentilactobacillus buchneri

M1GRN3

747208

sequence comparisons, recombinant expression of His/ProS2-tagged wild-type and mutant enzymes

Lentilactobacillus buchneri

M1GRN3

746630