Information on EC 5.1.1.21 - isoleucine 2-epimerase

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The expected taxonomic range for this enzyme is: Lactobacillus

EC NUMBER
COMMENTARY hide
5.1.1.21
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RECOMMENDED NAME
GeneOntology No.
isoleucine 2-epimerase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
L-isoleucine = D-allo-isoleucine
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
isoleucine 2-epimerase
A pyridoxal phosphate protein. The enzyme, characterized from the bacterium Lactobacillus buchneri, specifically catalyses racemization of nonpolar amino acids at the C-2 position.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(R)-2-aminobutyric acid
(S)-2-aminobutyric acid
show the reaction diagram
activity is 42% compared to D-allo-isoleucine
-
-
r
(S)-2-aminobutyric acid
(R)-2-aminobutyric acid
show the reaction diagram
activity is 32% compared to L-isoleucine
-
-
r
D-allo-isoleucine
L-isoleucine
show the reaction diagram
D-leucine
L-leucine
show the reaction diagram
activity is 30% compared to D-allo-isoleucine
-
-
?
D-methionine
L-methionine
show the reaction diagram
activity is 20% compared to D-allo-isoleucine
-
-
?
D-norleucine
L-norleucine
show the reaction diagram
activity is 59% compared to D-allo-isoleucine
-
-
r
D-norvaline
L-norvaline
show the reaction diagram
D-phenylalanine
L-phenylalanine
show the reaction diagram
activity is 25% compared to D-allo-isoleucine
-
-
?
D-valine
L-Val
show the reaction diagram
activity is 52% compared to D-allo-isoleucine
-
-
r
L-isoleucine
D-allo-isoleucine
show the reaction diagram
L-leucine
D-leucine
show the reaction diagram
activity is 30% compared to L-isoleucine
-
-
?
L-Methionine
D-Methionine
show the reaction diagram
L-Norleucine
D-Norleucine
show the reaction diagram
L-Norvaline
D-Norvaline
show the reaction diagram
L-phenylalanine
D-phenylalanine
show the reaction diagram
activity is 24% compared to L-isoleucine
-
-
?
L-valine
D-Val
show the reaction diagram
activity is 48% compared to L-isoleucine
-
-
r
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
hydroxylamine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13.2
D-allo-isoleucine
pH 5.5, 37°C
5
L-isoleucine
pH 5.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
939
D-allo-isoleucine
Lactobacillus buchneri
M1GRN3
pH 5.5, 37°C
502
L-isoleucine
Lactobacillus buchneri
M1GRN3
pH 5.5, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
71.4
D-allo-isoleucine
Lactobacillus buchneri
M1GRN3
pH 5.5, 37°C
43967
101
L-isoleucine
Lactobacillus buchneri
M1GRN3
pH 5.5, 37°C
311
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32
pH 5.5, 37°C, recombinant enzyme, substrate: L-methionine
34.3
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pH 5.5, 37°C
35.7
pH 5.5, 37°C, recombinant enzyme, substrate: L-phenylalanine
44.2
pH 5.5, 37°C, recombinant enzyme, substrate: L-leucine
44.5
pH 5.5, 37°C, recombinant enzyme, substrate: D-isoleucine
47.7
pH 5.5, 37°C, recombinant enzyme, substrate: (S)-2-aminobutyric acid
54.8
pH 5.5, 37°C, recombinant enzyme, substrate: D-leucine
66.3
pH 5.5, 37°C, recombinant enzyme, substrate: (R)-2-aminobutyric acid
71.7
pH 5.5, 37°C, recombinant enzyme, substrate: L-valine
74.1
pH 5.5, 37°C, recombinant enzyme, substrate: L-norleucine
83.4
pH 5.5, 37°C, recombinant enzyme, substrate: L-norvaline
93.7
pH 5.5, 37°C, recombinant enzyme, substrate: D-methionine
116
pH 5.5, 37°C, recombinant enzyme, substrate: D-norleucine
131
pH 5.5, 37°C, recombinant enzyme, substrate: D-valine
149
pH 5.5, 37°C, recombinant enzyme, substrate: L-isoleucine
183
pH 5.5, 37°C, recombinant enzyme, substrate: D-norvaline
221
pH 5.5, 37°C, recombinant enzyme, substrate: D-allo-isoleucine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
highest activity for the reaction with D-allo-L-isoleucine
50
highest activity for the reaction with L-isoleucine
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
4 * 49000, SDS-PAGE
200000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 11
the enzyme retains about 80% of its activity after incubation for 30 min
729935
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
30 min, the enzyme retains more than 80% of its activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli