4.5.1.3: dichloromethane dehalogenase

This is an abbreviated version!
For detailed information about dichloromethane dehalogenase, go to the full flat file.

Reaction

Synonyms

DCM dehalogenase, DcmA, dcmAa, DehalA, dehalogenase, dichloromethane, dichloromethane chloride-lyase, dichloromethane dehalogenase

ECTree

     4 Lyases

         4.5 Carbon-halide lyases

             4.5.1 Carbon-halide lyases (only sub-subclass identified to date)

                4.5.1.3 dichloromethane dehalogenase

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Results	in table
26	Activating Compound
120	AA Sequence
1	Application
1	CAS Registry Number
15	Cloned(Commentary)
12	Cofactor
5	Expression
23	Inhibitors
50	KM Value [mM]
1	Localization
9	Molecular Weight [Da]
43	Natural Substrates/ Products (Substrates)
134	Organism
2	Pathway
5	pH Optimum
1	pH Range
1	pH Stability
1	pI Value
21	Protein Variants
13	Purification (Commentary)
3	Reaction
1	Reaction Type
59	Reference
6	Source Tissue
16	Specific Activity [micromol/min/mg]
1	Storage Stability
94	Substrates and Products (Substrate)
9	Subunits
50	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
34	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 4.5.1.3 - dichloromethane dehalogenase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.112

Methylophilus sp.

P43387

W117Y with dichloromethane as substrate

648763

0.436

Methylophilus sp.

P43387

W117Y with diiodomomethane as substrate

648763

0.804

Methylophilus sp.

P43387

W117Y with CH2ClI as substrate

648763

0.914

Methylophilus sp.

P43387

W117Y with CH2BrCl as substrate

648763

0.98

Methylophilus sp.

P43387

W117Y with dibromomethane as substrate

648763

1.041

Hyphomicrobium sp.

-

hydroxylapatite eluate

648755

108.1

Niallia circulans

B7TXX4

-

702713

118.8

Niallia circulans

-

after purification by anion exchange column

671564

14.36

Niallia circulans

-

crude extract

671564

21.38

Niallia circulans

-

after purification by (NH4)2SO4 salting out

671564

3.336

Methylophilus sp.

P43387

wild-type with CH2BrCl as substrate

648763

3.618

Methylophilus sp.

P43387

wild-type with CH2ClI as substrate

648763

4.668

Methylophilus sp.

P43387

wild-type with dichloromethane as substrate

648763

4.728

Methylophilus sp.

P43387

wild-type with diiodomomethane as substrate

648763

49.12

Niallia circulans

-

after purification by Sephadex G-75 chromatography

671564

7.398

Methylophilus sp.

P43387

wild-type with dibromomethane as substrate

648763