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Literature summary for 4.5.1.3 extracted from

  • Cai, B.; Vuilleumier, S.; Wackett, L.P.
    Purification and characterization of the mutant enzyme W117Y of the dichloromethane dehalogenase from Methylophilus sp. strain DM11 (1998), Ann. N. Y. Acad. Sci., 864, 210-213.
    View publication on PubMed
Search for more literature for 4.5.1.3

Activating Compound

Activating Compound Comment Organism Structure
glutathione
-
 Methylophilus sp.

Cloned(Commentary)

Cloned (Comment) Organism
-
 Methylophilus sp.

Protein Variants

Protein Variants Comment Organism
W117Y higher KM and lower specific activity for all tested substrates, better heat stability than wild-type Methylophilus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 CH2BrCl pH 8.2, wild-type Methylophilus sp.
0.009
-
 CH2ClI pH 8.2, wild-type Methylophilus sp.
0.02
-
 Dibromomethane pH 8.2, wild-type Methylophilus sp.
0.055
-
 Diiodomethane pH 8.2, wild-type Methylophilus sp.
0.067
-
 Dichloromethane pH 8.2, wild-type Methylophilus sp.
0.18
-
 CH2ClI pH 8.2, W117Y Methylophilus sp.
0.338
-
 Dibromomethane pH 8.2, W117Y Methylophilus sp.
0.4
-
 Dichloromethane pH 8.2, W117Y Methylophilus sp.
0.43
-
 CH2BrCl pH 8.2, W117Y Methylophilus sp.
0.521
-
 Diiodomethane pH 8.2, W117Y Methylophilus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

Organism UniProt Comment Textmining
Methylophilus sp. P43387
-
-
Methylophilus sp. DM11 P43387
-
-

Purification (Commentary)

Purification (Comment) Organism
strain DM11 Methylophilus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.112
-
 W117Y with dichloromethane as substrate Methylophilus sp.
0.436
-
 W117Y with diiodomomethane as substrate Methylophilus sp.
0.804
-
 W117Y with CH2ClI as substrate Methylophilus sp.
0.914
-
 W117Y with CH2BrCl as substrate Methylophilus sp.
0.98
-
 W117Y with dibromomethane as substrate Methylophilus sp.
3.336
-
 wild-type with CH2BrCl as substrate Methylophilus sp.
3.618
-
 wild-type with CH2ClI as substrate Methylophilus sp.
4.668
-
 wild-type with dichloromethane as substrate Methylophilus sp.
4.728
-
 wild-type with diiodomomethane as substrate Methylophilus sp.
7.398
-
 wild-type with dibromomethane as substrate Methylophilus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH2BrCl + H2O
-
 Methylophilus sp. formaldehyde + Br- + Cl-
-
 ?
CH2BrCl + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br- + Cl-
-
 ?
CH2ClI + H2O
-
 Methylophilus sp. formaldehyde + Cl- + I-
-
 ?
CH2ClI + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl- + I-
-
 ?
dibromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
dibromomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?
diiodomethane + H2O
-
 Methylophilus sp. formaldehyde + I-
-
 ?
diiodomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + I-
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 after 5 min remains 38% activity of wild-type enzyme and 54% activity of W117Y Methylophilus sp.