4.5.1.3: dichloromethane dehalogenase
This is an abbreviated version!
For detailed information about dichloromethane dehalogenase, go to the full flat file.
Word Map on EC 4.5.1.3
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4.5.1.3
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s-transferase
-
methylotrophic
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methylophilus
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theta
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dehalogenation
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extorquens
-
hyphomicrobium
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dichloromethanicum
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transconjugants
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dihalomethanes
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glutathione-dependent
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methylobacteria
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circulans
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degradation
- 4.5.1.3
- s-transferase
-
methylotrophic
- methylophilus
- theta
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dehalogenation
- extorquens
- hyphomicrobium
- dichloromethanicum
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transconjugants
-
dihalomethanes
-
glutathione-dependent
-
methylobacteria
- circulans
- degradation
Reaction
Synonyms
DCM dehalogenase, DcmA, dcmAa, DehalA, dehalogenase, dichloromethane, dichloromethane chloride-lyase, dichloromethane dehalogenase
ECTree
4.5.1.3 dichloromethane dehalogenaseAdvanced search results
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results (Engineering
Engineering on EC 4.5.1.3 - dichloromethane dehalogenase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
H8F
little effect on the activity, but slower turnover of dibromomethane than wild-type
H8L
little effect on the activity, but slower turnover of dibromomethane than wild-type
H8Y
little effect on the activity, but slower turnover of dibromomethane than wild-type
V18A
lower affinity for GSH and slower turnover of dichlormethane than wild-type
V18I
lower affinity for GSH and slower turnover of dichlormethane than wild-type
V18T
lower affinity for GSH and slower turnover of dichlormethane than wild-type
W117Y
higher KM and lower specific activity for all tested substrates, better heat stability than wild-type
H8F
-
little effect on the activity, but slower turnover of dibromomethane than wild-type
-
H8L
-
little effect on the activity, but slower turnover of dibromomethane than wild-type
-
V18A
-
lower affinity for GSH and slower turnover of dichlormethane than wild-type
-
V18I
-
lower affinity for GSH and slower turnover of dichlormethane than wild-type
-
V18T
-
lower affinity for GSH and slower turnover of dichlormethane than wild-type
-
W117Y
-
higher KM and lower specific activity for all tested substrates, better heat stability than wild-type
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additional information
additional information
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme
additional information
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme
additional information
generation of a METDI2657 gene knockout mutant strain NOK353 from Methylobacterium dichloromethanicum wild-type strain DM4 using the mobilized suicide vector pK18mob, the mutant strain shows reduced growth on methanol compared to the wild-type enzyme
