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Literature summary for 4.5.1.3 extracted from

  • Wu, S.J.; Zhang, L.L.; Wang, J.D.; Chen, J.M.
    Bacillus circulans WZ-12 - a newly discovered aerobic dichloromethane-degrading methylotrophic bacterium (2007), Appl. Microbiol. Biotechnol., 76, 1289-1296.
    View publication on PubMed
Search for more literature for 4.5.1.3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 estimated by SDS-PAGE Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dichloromethane + H2O Niallia circulans
-
 formaldehyde + chloride
-
 ?
dichloromethane + H2O Methylobacterium sp.
-
 formaldehyde + chloride
-
 ?
dichloromethane + H2O Hyphomicrobium sp. DM2
-
 formaldehyde + chloride
-
 ?
dichloromethane + H2O Methylorubrum extorquens DM4
-
 formaldehyde + chloride
-
 ?
dichloromethane + H2O Niallia circulans WZ12
-
 formaldehyde + chloride
-
 ?
dichloromethane + H2O Methylobacterium sp. DM11
-
 formaldehyde + chloride
-
 ?

Organism

Organism UniProt Comment Textmining
Hyphomicrobium sp. DM2
-
-
-
Methylobacterium sp.
-
 DM11
-
Methylobacterium sp. DM11
-
 DM11
-
Methylorubrum extorquens DM4
-
-
-
Niallia circulans
-
-
-
Niallia circulans WZ12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to 8.27-fold with a yield of 34.83%, purified by ammonium sulfate fractionation, gel filtration chromatography and anion-exchange chromatography Niallia circulans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.36
-
 crude extract Niallia circulans
21.38
-
 after purification by (NH4)2SO4 salting out Niallia circulans
49.12
-
 after purification by Sephadex G-75 chromatography Niallia circulans
118.8
-
 after purification by anion exchange column Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dichloromethane + H2O
-
 Niallia circulans formaldehyde + chloride
-
 ?
dichloromethane + H2O
-
 Methylobacterium sp. formaldehyde + chloride
-
 ?
dichloromethane + H2O
-
 Hyphomicrobium sp. DM2 formaldehyde + chloride
-
 ?
dichloromethane + H2O
-
 Methylorubrum extorquens DM4 formaldehyde + chloride
-
 ?
dichloromethane + H2O
-
 Niallia circulans WZ12 formaldehyde + chloride
-
 ?
dichloromethane + H2O
-
 Methylobacterium sp. DM11 formaldehyde + chloride
-
 ?

Synonyms

Synonyms Comment Organism
dichloromethane dehalogenase
-
 Niallia circulans
dichloromethane dehalogenase
-
 Methylobacterium sp.
dichloromethane dehalogenase
-
 Hyphomicrobium sp. DM2
dichloromethane dehalogenase
-
 Methylorubrum extorquens DM4

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.55
-
 Dichloromethane pH 8.0 and 30°C Hyphomicrobium sp. DM2
0.87
-
 Dichloromethane pH 8.0 and 30°C Methylorubrum extorquens DM4
3.28
-
 Dichloromethane pH 8.0 and 30°C Methylobacterium sp.
3.3
-
 Dichloromethane pH 8.0 and 30°C Niallia circulans
4.78
-
 Dichloromethane pH 8.0 and 30°C Niallia circulans

Cofactor

Cofactor Comment Organism Structure
glutathione strictly required Niallia circulans
glutathione strictly required Methylobacterium sp.
glutathione strictly required Hyphomicrobium sp. DM2
glutathione strictly required Methylorubrum extorquens DM4