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4.3.1.2: methylaspartate ammonia-lyase

This is an abbreviated version!
For detailed information about methylaspartate ammonia-lyase, go to the full flat file.

Word Map on EC 4.3.1.2

Reaction

L-threo-3-methylaspartate
=
mesaconate
 +
NH3

Synonyms

3-methyl-L-aspartic acid ammonia-lyase, 3-methylaspartase, 3-methylaspartate ammonia lyase, 3-methylaspartate ammonia-lyase, ammonia-lyase, methylaspartate, beta-methylaspartase, Ch-MAL, MAL, methylaspartate ammonia lyase, methylaspartate ammonia-lyase, threo-3-methyl-L-aspartate ammonia-lyase, threo-3-methylaspartase ammonia-lyase

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.1 Ammonia-lyases
                4.3.1.2 methylaspartate ammonia-lyase

Purification

Purification on EC 4.3.1.2 - methylaspartate ammonia-lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
from whole cell lysates for protein identification using 2D-electrophoresis and LC-MS/MS
-
heat, ammonium sulfate, gel filtration, DEAE-Sepharose, ethanol
-
JCM 1658, JCM 1659
-
native and recombinant 3-methylaspartase
-
recombinant 3-methylaspartase
using metal affinity chromatography
YG-0504, TPU 0011, IFO 13539, IFO 13545, IFO 13546
-
YG-0601, IFO 3168, IFO 3848, NCIMB 232, NCIMB 9525, NCIMB 10466, NCTC 1707, NCTC 2815, NCTC 2818, NCTC 5845, NCTC 7381, NCTC10011, NCTC 10375, NCTC11354, NCTC 12028
-
YG-1002, IFO 13547
-