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Literature summary for 4.3.1.2 extracted from

  • Bearne, S.L.; White, R.L.; MacDonnell, J.E.; Bahrami, S.; Gronlund, J.
    Purification and characterization of beta-methylaspartase from Fusobacterium varium (2001), Mol. Cell. Biochem., 221, 117-126.
    View publication on PubMed
Search for more literature for 4.3.1.2

Activating Compound

Activating Compound Comment Organism Structure
NH4+ approx. 65% of K+ activation Fusobacterium varium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
 (2S,3S)-methylaspartate 25°C, pH 9.7 Fusobacterium varium
1.5
-
 (S)-aspartate 25°C, pH 9.7 Fusobacterium varium

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ approx. 5% of Mg2+ activation Fusobacterium varium
Cu2+ approx. 10% of Mg2+ activation Fusobacterium varium
Fe2+ approx. 5% of Mg2+ activation Fusobacterium varium
K+ required for activity Fusobacterium varium
Li+ approx. 20% of K+ activation Fusobacterium varium
Mg2+ required for activity Fusobacterium varium
Mn2+ approx. 40% of Mg2+ activation Fusobacterium varium
Na+ approx. 7% of K+ activation Fusobacterium varium
Ni2+ approx. 10% of Mg2+ activation Fusobacterium varium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 2 * 46000, SDS-PAGE Fusobacterium varium
88000
-
 gel filtration Fusobacterium varium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threo-3-methylaspartate Fusobacterium varium
-
 mesaconate + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Fusobacterium varium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heat, ammonium sulfate, gel filtration, DEAE-Sepharose, ethanol Fusobacterium varium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
137
-
-
 Fusobacterium varium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate
-
 Fusobacterium varium fumarate + NH3
-
 ?
L-threo-3-methylaspartate
-
 Fusobacterium varium mesaconate + NH3
-
 r
NH3 + (2E)-2-methylbut-2-enedioate
-
 Fusobacterium varium (2S,3S)-3-methylaspartate major product r
NH3 + (2E)-2-methylbut-2-enedioate
-
 Fusobacterium varium (2S,3R)-3-methylaspartate minor product r

Subunits

Subunits Comment Organism
dimer 2 * 46000, SDS-PAGE Fusobacterium varium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
 amination Fusobacterium varium
9.7
-
 deamination Fusobacterium varium

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11 55% of maximal amination activity at pH 8.5, 75% of maximal amination activity at pH 11.0, 30% of maximal deamination activity at pH 8.5, 15% of maximal deamination activity at pH 11.0 Fusobacterium varium