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Literature summary for 4.3.1.2 extracted from

  • Raj, H.; Puthan Veetil, V.; Szymanski, W.; Dekker, F.J.; Quax, W.J.; Feringa, B.L.; Janssen, D.B.; Poelarends, G.J.
    Characterization of a thermostable methylaspartate ammonia lyase from Carboxydothermus hydrogenoformans (2012), Appl. Microbiol. Biotechnol., 94, 385-397.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.1.2

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Carboxydothermus hydrogenoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6
-
 (2S,3S)-3-ethylaspartic acid pH 9, 30°C Carboxydothermus hydrogenoformans
16
-
 (2S,3S)-3-ethylaspartic acid pH 9, 70°C Carboxydothermus hydrogenoformans

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required for maximum activity Carboxydothermus hydrogenoformans
Mg2+ required for maximum activity Carboxydothermus hydrogenoformans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 SDS-PAGE Carboxydothermus hydrogenoformans

Organism

Organism UniProt Comment Textmining
Carboxydothermus hydrogenoformans Q3AEU2
-
-

Purification (Commentary)

Purification (Comment) Organism
using metal affinity chromatography Carboxydothermus hydrogenoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-3-ethylaspartic acid
-
 Carboxydothermus hydrogenoformans ?
-
 ?
ethylfumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
fumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
mesaconate + ethylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
mesaconate + hydrazine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
mesaconate + hydroxylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
mesaconate + methoxylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
mesaconate + methylamine
-
 Carboxydothermus hydrogenoformans ?
-
 ?
mesaconate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
methylfumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?
propylfumarate + NH3
-
 Carboxydothermus hydrogenoformans ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 49000 Carboxydothermus hydrogenoformans

Synonyms

Synonyms Comment Organism
Ch-MAL
-
 Carboxydothermus hydrogenoformans
methylaspartate ammonia lyase
-
 Carboxydothermus hydrogenoformans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Carboxydothermus hydrogenoformans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable for more than 4 hours Carboxydothermus hydrogenoformans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16
-
 (2S,3S)-3-ethylaspartic acid pH 9, 30°C Carboxydothermus hydrogenoformans
78
-
 (2S,3S)-3-ethylaspartic acid pH 9, 70°C Carboxydothermus hydrogenoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Carboxydothermus hydrogenoformans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.9
-
 (2S,3S)-3-ethylaspartic acid pH 9, 30°C Carboxydothermus hydrogenoformans
35
-
 (2S,3S)-3-ethylaspartic acid pH 9, 70°C Carboxydothermus hydrogenoformans