4.3.1.2: methylaspartate ammonia-lyase
This is an abbreviated version!
For detailed information about methylaspartate ammonia-lyase, go to the full flat file.
Word Map on EC 4.3.1.2
-
4.3.1.2
-
seizure
-
grand
-
epilepsy
-
petit
-
children
-
convulsion
-
pain
-
discharge
-
anticonvulsant
-
photodynamic
-
myoclonic
-
woman
-
electroencephalograph
-
allergen
-
aminolevulinate
-
antiepileptic
-
wave
-
awaken
-
palmoplantar
-
diazepam
-
phenytoin
-
tonic-clonic
-
arcuate
-
celiac
-
cream
-
rehabilitation
-
hemiparesis
-
consciousness
-
carbamazepine
-
ligament
-
paroxysm
-
birch
-
epilepticus
-
seizure-free
-
electroshock
-
clonazepam
-
jerk
-
psychogenic
-
analysis
-
reoviridae
-
ictal
-
keratoses
-
trif
-
maackia
-
amurensis
-
interictal
-
clonic
-
synthesis
-
biotechnology
-
diphenylhydantoin
-
sway
-
daylight
-
wolf
- 4.3.1.2
- seizure
-
grand
- epilepsy
-
petit
- children
- convulsion
- pain
- discharge
-
anticonvulsant
-
photodynamic
-
myoclonic
- woman
-
electroencephalograph
- allergen
-
aminolevulinate
-
antiepileptic
-
wave
-
awaken
-
palmoplantar
- diazepam
- phenytoin
-
tonic-clonic
-
arcuate
-
celiac
-
cream
-
rehabilitation
- hemiparesis
- consciousness
- carbamazepine
-
ligament
-
paroxysm
- birch
- epilepticus
-
seizure-free
-
electroshock
- clonazepam
- jerk
-
psychogenic
- analysis
- reoviridae
-
ictal
- keratoses
- trif
-
maackia
- amurensis
-
interictal
-
clonic
- synthesis
- biotechnology
- diphenylhydantoin
-
sway
-
daylight
- wolf
Reaction
Synonyms
3-methyl-L-aspartic acid ammonia-lyase, 3-methylaspartase, 3-methylaspartate ammonia lyase, 3-methylaspartate ammonia-lyase, ammonia-lyase, methylaspartate, beta-methylaspartase, Ch-MAL, MAL, methylaspartate ammonia lyase, methylaspartate ammonia-lyase, threo-3-methyl-L-aspartate ammonia-lyase, threo-3-methylaspartase ammonia-lyase
ECTree
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Substrates Products
Substrates Products on EC 4.3.1.2 - methylaspartate ammonia-lyase
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REACTION DIAGRAM
(2E)-2-butylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 59%
-
-
r
(2E)-2-ethylbut-2-enedioate + NH3
?
wild-type: conversion 74%, mutant L384A: conversion 76%
-
-
r
(2E)-2-hexylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 53%
-
-
r
(2E)-2-methylbut-2-enedioate + NH3
?
wild-type: conversion 80%, mutant L384A: conversion 75%
-
-
r
(2E)-2-pentylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 52%
-
-
r
(2E)-2-phenylbut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 90%
-
-
r
(2E)-2-propylbut-2-enedioate + NH3
?
wild-type: conversion 57%, mutant L384A: conversion 67%
-
-
r
(2S,3S)-N-hydroxy-3-ethylaspartic acid
ethylfumarate + hydroxylamine
-
-
-
?
(2S,3S)-N-hydroxy-3-methylaspartic acid
mesaconate + hydroxylamine
-
-
-
?
(2S,3S)-N-methoxy-3-methylaspartic acid
mesaconate + methoxylamine
-
-
-
?
(2Z)-2-(benzyloxy)but-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 60%
-
-
r
(2Z)-2-(benzylsulfanyl)but-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 42%
-
-
r
(2Z)-2-(ethylsulfanyl)but-2-enedioate + NH3
?
wild-type: conversion 36%, mutant L384A: conversion 50%
-
-
r
(2Z)-2-(phenylsulfanyl)but-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 34%
-
-
r
(2Z)-2-ethoxybut-2-enedioate + NH3
?
wild-type: conversion 46%, mutant L384A: conversion 43%
-
-
r
(2Z)-2-phenoxybut-2-enedioate + NH3
?
wild-type: conversion 0%, mutant L384A: conversion 44%
-
-
r
2-hydrazino-3-isopropylsuccinic acid
isopropylfumarate + hydrazine
-
-
-
?
3-fluoroaspartate
fluorofumarate + NH3
-
-
not a good substrate
?
L-threo-3-methylaspartic acid
mesaconate + NH3
assay at pH 9.0, 30°C
-
-
?
mesaconate + (aminooxy)ethane
?
mutant Q73A, conversion: 100%, yield: 61%
-
-
r
mesaconate + (aminooxy)methane
?
mutant Q73A, conversion: 99%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + 2-aminoethanol
?
mutant Q73A, conversion: 37%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + aniline
?
mutant Q73A, conversion: 6%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + cyclohexanamine
?
mutant Q73A, conversion: 21%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + cyclopentanamine
?
mutant Q73A, conversion: 36%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + hexanamine
?
mutant Q73A, conversion: 17%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + N-methylethane-1,2-diamine
?
mutant Q73A, conversion: 80%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate + L-erythro-3-methylaspartate
conversion 2h: 73% (wild-type), diastereomeric product ratio (threo:erythro) after 2h: 89:11
-
-
r
mesaconate + propan-2-amine
?
mutant Q73A, conversion: 8%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconic acid + NH3
L-threo-3-methylaspartate
assay at pH 9.0, 30°C
-
-
r
NH3 + (2E)-2-methylbut-2-enedioate
(2S,3R)-3-methylaspartate
-
-
minor product
r
NH3 + (2E)-2-methylbut-2-enedioate
(2S,3S)-3-methylaspartate
-
-
major product
r
(2R,3S)-3-chloroaspartic acid
2-chloro-2-butenedioic acid
-
-
-
-
?
3-bromoaspartate
bromofumarate + NH3
-
-
during reaction, enzyme becomes inactive. This is reduced in the presence of alternative substrates
?
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
3-chloroaspartate
chlorofumarate + NH3
-
(2R,3S)-3-chloroaspartic acid
-
?
erythro-3-methylaspartate
?
-
(2S,3R)-3-methylaspartic acid
-
-
?
erythro-3-methylaspartate
?
Clostridium tetanomorphum H1 / ATCC 15920
-
erythro-3-methyl-L-aspartate
-
-
?
erythro-3-methylaspartate
?
Clostridium tetanomorphum H1 / ATCC 15920
-
erythro-beta-methylaspartate
-
-
?
L-aspartate
fumarate + NH3
-
rate of deamination 100 times slower than that of L-threo-3-methylaspartate
-
?
L-aspartate
fumarate + NH3
-
(2S,3R) [3-3H]aspartic acid
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
-
(2S)-aspartate
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
rate of deamination 100 times slower than that of L-threo-3-methylaspartate
-
?
L-aspartate
fumarate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S,3R) [3-3H]aspartic acid
amination 1.8 times faster than the amination of mesaconate
?
L-aspartate
fumarate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S)-aspartate
amination 1.8 times faster than the amination of mesaconate
?
mesaconate + NH3
-
i.e. (2S,3R)-3-methylaspartate, slow syn elimination
-
?
L-erythro-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3R)-3-methylaspartate, slow syn elimination
-
?, r
L-threo-3-methylaspartate
?
-
-
5670, 5997, 6049, 6050, 6051, 6053, 6054, 6055, 6056, 6057, 6058, 6059, 6060, 6062, 6064, 6065, 6066, 6067
-
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
beta-methylaspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3S)-3-methylaspartate
-
r
L-threo-3-methylaspartate
mesaconate + NH3
-
i.e. (2S,3S)-3-methylaspartate
-
r
L-threo-3-methylaspartate
mesaconate + NH3
the enzyme is part of a catabolic pathway for L-glutamate
-
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
i.e. (2S,3S)-3-methylaspartate
-
r
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
threo-beta-methyl-L-aspartate
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
Clostridium tetanomorphum H1 / ATCC 15920
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
L-threo-3-methylaspartate
mesaconate + NH3
-
(2S,3S)-3-methylaspartic acid
-
?
?
mutant Q73A, conversion: 61%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + 1-cyclopropylmethanamine
?
mutant Q73A, conversion: 96%, yield: 28%, enantiomeric excess: more than 99%
-
-
r
?
mutant Q73A, conversion: 100%, yield: 53%
-
-
r
mesaconate + 2-methoxyethanamine
?
mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
?
mutant Q73A, conversion: 56%, excess of threo isomer over erythro isomer: 80%
-
-
r
mesaconate + 3-aminopropan-1-ol
?
mutant Q73A, conversion: 99%, yield: 57%
-
-
r
?
mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + aminobutane
?
mutant Q73A, conversion: 97%, yield: 33%, enantiomeric excess: more than 99%
-
-
r
?
mutant Q73A, conversion: 65%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + cyclobutanamine
?
mutant Q73A, conversion: 99%, yield: 74%, enantiomeric excess: more than 99%
-
-
r
?
mutant Q73A, conversion: 100%, yield: 48%
-
-
r
mesaconate + cyclopropanamine
?
mutant Q73A, conversion: 75%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
?
mutant Q73A, conversion: 100%, yield: 75%, enantiomeric excess: more than 99%
-
-
r
mesaconate + ethanamine
?
mutant Q73A, conversion: 51%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
?
mutant Q73A, conversion: 90%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + ethane-1,2-diamine
?
mutant Q73A, conversion: 99%, yield: 67%
-
-
r
?
mutant Q73A, conversion: 100%, yield: 96%, enantiomeric excess: more than 99%
-
-
r
mesaconate + methanamine
?
mutant Q73A, conversion: 74%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
mutant Q73A, conversion: 100%, yield: 79%, enantiomeric excess: more than 99%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
mutant Q73A, conversion: 79%, excess of threo isomer over erythro isomer: 5%
-
-
r
mesaconate + NH3
L-threo-3-methylaspartate
wild-type: conversion 100%, mutant L384A: conversion 99%
-
-
r
?
mutant Q73A, conversion: 29%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
mesaconate + pentanamine
?
mutant Q73A, conversion: 91%, yield: 14%, enantiomeric excess: more than 99%
-
-
r
?
mutant Q73A, conversion: 53%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99%
-
-
r
mesaconate + propanamine
?
mutant Q73A, conversion: 99%, yield: 53%, enantiomeric excess: more than 99%
-
-
r
?
mutant Q73A, conversion: 100%, yield: 61%
-
-
r
mesaconate + propane-1,3-diamine
?
mutant Q73A, conversion: 78%, excess of threo isomer over erythro isomer: more than 95%
-
-
r
additional information
?
-
-
no reaction with aspartic acid
-
-
?
additional information
?
-
-
enzyme is specific for the L-configuration
-
-
?
additional information
?
-
-
catalytic rates for the enzymatic amination of fumarate and mesaconate are faster than the rates of the corresponding deamination reactions
-
-
?
additional information
?
-
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
-
-
?
additional information
?
-
Clostridium tetanomorphum H1 / ATCC 15920
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
Clostridium tetanomorphum H1 / ATCC 15920
-
enzyme is involved in the catabolic pathway for glutamate
-
-
?
additional information
?
-
-
enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes
-
-
?
additional information
?
-
-
ammonia is the natural nucleophile for the enzyme
-
-
?