4.2.3.57: (-)-beta-caryophyllene synthase
This is an abbreviated version!
For detailed information about (-)-beta-caryophyllene synthase, go to the full flat file.
Word Map on EC 4.2.3.57
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4.2.3.57
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sesquiterpene
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terpene
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e-beta-caryophyllene
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enemies
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diabrotica
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herbivore-induced
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virgifera
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biofuel production
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agriculture
- 4.2.3.57
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sesquiterpene
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terpene
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e-beta-caryophyllene
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enemies
- diabrotica
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herbivore-induced
- virgifera
- biofuel production
- agriculture
Reaction
Synonyms
(E)-beta-caryophyllene synthase, alpha-humulene/(-)-(E)-beta-caryophyllene synthase, At5g23960, BCS, beta-caryophyllene synthase, CarS, E-(beta)-caryophyllene synthase, EbetaC synthase, GwECar2, More, OkBCS, PnCPS, PnTPS1, QHS1, RtTPS1, RtTPS3, TPS04, TPS1, TPS21, tps23, TPS3
ECTree
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Substrates Products
Substrates Products on EC 4.2.3.57 - (-)-beta-caryophyllene synthase
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REACTION DIAGRAM
(2E,6E)-farnesyl diphosphate
(-)-beta-caryophyllene + alpha-humulene + diphosphate
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(2E,6E)-farnesyl diphosphate
(E)-beta-caryophyllene + alpha-humulene + alpha-copaene + diphosphate
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alpha-humulene and alpha-copaene are minor products besides the main product (E)-beta-caryophyllene
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?
(2E,6E)-farnesyl diphosphate
(E)-beta-caryophyllene + alpha-humulene + diphosphate
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TPS 1 forms two main products: (E)-beta-caryophyllene and alpha-humulene with 76.3 and 23.7%, respectively
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(2E,6E)-farnesyl diphosphate
(E)-beta-caryophyllene + alpha-humulene + germacrene + diphosphate
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?
(2E,6E)-farnesyl diphosphate
(trans)-beta-caryophyllene + diphosphate
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Geranyl diphosphate
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production of several monoterpenes among which myrcene, limonene and camphene can be clearly identified
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(-)-beta-caryophyllene + diphosphate
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(2E,6E)-farnesyl diphosphate
(-)-beta-caryophyllene + diphosphate
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?
(2E,6E)-farnesyl diphosphate
(-)-beta-caryophyllene + diphosphate
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?
(E)-beta-caryophyllene + diphosphate
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79% (E)-beta-caryophyllene plus 21% alpha-humulene
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(2E,6E)-farnesyl diphosphate
(E)-beta-caryophyllene + diphosphate
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beta-caryophyllene + diphosphate
stereochemistry not specified in the publication
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(2E,6E)-farnesyl diphosphate
beta-caryophyllene + diphosphate
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enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29
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(2E,6E)-farnesyl diphosphate
beta-caryophyllene + diphosphate
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(2E,6E)-farnesyl diphosphate
beta-caryophyllene + diphosphate
the enzyme stereochemistry is not determined in the publication. Recombinant enzyme OkBCS converts farnesyl diphosphate to beta-caryophyllene as a major product (94%) and 6% alpha-humulene
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(2E,6E)-farnesyl diphosphate
beta-caryophyllene + diphosphate
the enzyme stereochemistry is not determined in the publication
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(2E,6E)-farnesyl diphosphate
beta-caryophyllene + diphosphate
the enzyme stereochemistry is not determined in the publication, the reaction mechanism includes a 1,10-ring closure
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(2E,6E)-farnesyl diphosphate
beta-caryophyllene + diphosphate
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additional information
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enzyme OkBCS is not active with geranyl diphosphate (GPP) as substrate, besides a weak hydrolysis activity converting GPP to geraniol, identification of substrates and products by GC-MS analysis
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additional information
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enzyme OkBCS is not active with geranyl diphosphate (GPP) as substrate, besides a weak hydrolysis activity converting GPP to geraniol, identification of substrates and products by GC-MS analysis
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additional information
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the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS)
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additional information
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steady-state kinetic parameters are determined using malachite green phosphate assay
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