4.2.3.57: (-)-beta-caryophyllene synthase

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For detailed information about (-)-beta-caryophyllene synthase, go to the full flat file.

Reaction

Synonyms

(E)-beta-caryophyllene synthase, alpha-humulene/(-)-(E)-beta-caryophyllene synthase, At5g23960, BCS, beta-caryophyllene synthase, CarS, E-(beta)-caryophyllene synthase, EbetaC synthase, GwECar2, More, OkBCS, PnCPS, PnTPS1, QHS1, RtTPS1, RtTPS3, TPS04, TPS1, TPS21, tps23, TPS3

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.3 Acting on phosphates

                4.2.3.57 (-)-beta-caryophyllene synthase

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Results	in table
28	AA Sequence
3	Application
14	Cloned(Commentary)
4	Expression
11	General Information
2	kcat/KM [mM/s]
6	KM Value [mM]
3	Localization
1	Metals/Ions
1	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
18	Organism
4	Pathway
9	PDB ID
2	pH Optimum
1	pI Value
4	Protein Variants
5	Purification (Commentary)
2	Reaction
16	Reference
20	Source Tissue
23	Substrates and Products (Substrate)
3	Subunits
33	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
3	Turnover Number [1/s]

Purification

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Purification on EC 4.2.3.57 - (-)-beta-caryophyllene synthase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Ni-IDA-Sepharose column chromatography

Rhodomyrtus tomentosa

A0A385J6X8, A0A385J6Y9

747883

Profinity IMAC resin column chromatography

Vitis vinifera

E5GAF4

748884

recombinant C-terminally His6-tagged enzyme from Escherichia coli TOP10 cells by nickel affinity chromatography to homogeneity

Ocimum kilimandscharicum

A0A0E3KJK7

746974

recombinant His-tagged enzyme TPS1 from Escherichia coli strain Rosetta 2(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration

Piper nigrum

A0A1V0E492

746924

recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Rhodomyrtus tomentosa

A0A385J6X8, A0A385J6Y9

747883