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4.2.3.57: (-)-beta-caryophyllene synthase

This is an abbreviated version!
For detailed information about (-)-beta-caryophyllene synthase, go to the full flat file.

Word Map on EC 4.2.3.57

Reaction

(2E,6E)-farnesyl diphosphate
=
(-)-beta-caryophyllene
 +
diphosphate

Synonyms

(E)-beta-caryophyllene synthase, alpha-humulene/(-)-(E)-beta-caryophyllene synthase, At5g23960, BCS, beta-caryophyllene synthase, CarS, E-(beta)-caryophyllene synthase, EbetaC synthase, GwECar2, More, OkBCS, PnCPS, PnTPS1, QHS1, RtTPS1, RtTPS3, TPS04, TPS1, TPS21, tps23, TPS3

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.57 (-)-beta-caryophyllene synthase

Cloned

Cloned on EC 4.2.3.57 - (-)-beta-caryophyllene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
codon-optimized QHS1 gene, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH
expressed in Escherichia coli BL21 (DH3) pLysS cells
expressed in Escherichia coli BL21 cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21, Rosetta and RIPL strains and in Arabidopsis thaliana
expressed in Escherichia coli strain YJM59
gene OkBCS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli TOP10 cells, transient overexpression in Ocimum kilimandscharicum via Agrobacterium tumefaciens strain GV3101 mediated transfection
gene QHS1, the optimized QHS1 gene is expressed in Escherichia coli strain YJM63 and BL21(DE3), coexpression with genes ACS, GPPS2, and AACS to construct a beta-caryophyllene overroduction strain
gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS), illumina transcriptome sequencing of unripe peppercorn identifiying sesquiterpene synthases (sesqui-TPSs), recombinant expression of His-tagged enzyme TPS1 in Escherichia coli strain Rosetta 2(DE3)
gene TPS1, sequence comparisons and phylogenetic tree, isozyme TPS1 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)
gene TPS21, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH
gene TPS3, sequence comparisons and phylogenetic tree, isozyme TPS3 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3)