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4.2.3.131: miltiradiene synthase

This is an abbreviated version!
For detailed information about miltiradiene synthase, go to the full flat file.

Word Map on EC 4.2.3.131

Reaction

(+)-copalyl diphosphate
=
miltiradiene
 +
diphosphate

Synonyms

ent-kaurene synthase-like, IrTPS4, kaurene synthase-like, kaurene synthase-like 1, kaurene synthase-like 2, KSL, KSL1, KSL2, KSL3, RoSKL1, RoSKL2, SfKSL, SmiKSL, SmMDS, TPS4, TwTPS27v2

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.3 Acting on phosphates
                4.2.3.131 miltiradiene synthase

Application

Application on EC 4.2.3.131 - miltiradiene synthase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
expression of copalyl diphosphate synthase and miltiradiene synthase in Saccharomyces cerevisiae, results in production of 4.2 mg/l miltiradiene. Miltiradiene production increases to 8.8 mg/l by improving supply of geranylgeranyl diphosphate by over-expression of a fusion gene of farnesyl diphosphate synthase ERG20 and endogenous geranylgeranyl diphosphate synthase BTS1 together with a heterologous geranylgeranyl diphosphate synthase from Sulfolobus acidocaldarius. Combinatorial overexpression of truncated 3-hydroxyl-3-methylglutaryl-CoA reductase with a mutated global regulatory factor upc2.1 and ERG20-BTS1-geranylgeranyl diphosphate synthase genes has synergetic effects on miltiradiene production, increasing titer to 61.8 mg/l. In fed-batch fermentation 488 mg/l miltiradiene can be produced