4.2.3.131: miltiradiene synthase
This is an abbreviated version!
For detailed information about miltiradiene synthase, go to the full flat file.
Word Map on EC 4.2.3.131
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4.2.3.131
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diterpenoids
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salvia
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diterpene
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tanshinones
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miltiorrhiza
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synthases
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copalyl
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triptolide
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wilfordii
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tripterygium
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hairy
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carnosol
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glandular
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ent-cpp
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lamiaceae
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jasmonate
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labdane-related
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cyclases
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ferruginol
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benthamiana
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trichome
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cosy
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one-hybrid
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synthesis
- 4.2.3.131
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diterpenoids
- salvia
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diterpene
- tanshinones
- miltiorrhiza
- synthases
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copalyl
- triptolide
- wilfordii
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tripterygium
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hairy
- carnosol
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glandular
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ent-cpp
- lamiaceae
- jasmonate
-
labdane-related
- cyclases
- ferruginol
- benthamiana
- trichome
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cosy
-
one-hybrid
- synthesis
Reaction
Synonyms
ent-kaurene synthase-like, IrTPS4, kaurene synthase-like, kaurene synthase-like 1, kaurene synthase-like 2, KSL, KSL1, KSL2, KSL3, RoSKL1, RoSKL2, SfKSL, SmiKSL, SmMDS, TPS4, TwTPS27v2
ECTree
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Substrates Products
Substrates Products on EC 4.2.3.131 - miltiradiene synthase
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REACTION DIAGRAM
8-hydroxy-(+)-copalyl diphosphate
manoyl oxide + diphosphate
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miltiradiene + diphosphate
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(+)-copalyl diphosphate
miltiradiene + diphosphate
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the enzyme is quite specific in its production of miltiradiene with more than 95% of the total product output
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(+)-copalyl diphosphate
miltiradiene + diphosphate
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products are abietatriene and miltiradiene, ratio of miltiradiene to abietatriene is 100:30. Miltiradiene spontaneously reacts to abietatriene
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(+)-copalyl diphosphate
miltiradiene + diphosphate
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synthesis of miltiradiene through the enzyme is confirmed by 1D and 2D NMR analyses, GC-MS analysis, overview
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additional information
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synthesis of miltiradiene through the enzyme is confirmed by 1D and 2D NMR analyses, GC-MS analysis, overview
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additional information
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MDS is a bifunctional miltiradiene synthase catalyzing the cyclization reaction of geranylgeranyl diphosphate via (+)-copalyl diphosphate to afford miltiradiene as a final product
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additional information
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substrate specificity analysis, rosemary RoKSL1/2 are functional enzymes able to convert either 8-OH-CDP or CDP into manoyl oxide or miltiradiene, respectively, and that they likely function in planta as miltiradiene synthases
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additional information
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substrate specificity analysis, rosemary RoKSL1/2 are functional enzymes able to convert either 8-OH-CDP or CDP into manoyl oxide or miltiradiene, respectively, and that they likely function in planta as miltiradiene synthases
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additional information
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miltiradiene spontaneously forms abietatriene
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additional information
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miltiradiene spontaneously forms abietatriene
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additional information
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GC-MS analysis of TwTPS27 assay product
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