Information on EC 4.2.3.131 - miltiradiene synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Mentheae

EC NUMBER
COMMENTARY hide
4.2.3.131
-
RECOMMENDED NAME
GeneOntology No.
miltiradiene synthase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(+)-copalyl diphosphate = miltiradiene + diphosphate
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
carnosate bioynthesis
-
-
Diterpenoid biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
(+)-copaly-diphosphate diphosphate-lyase (cyclizing, miltiradiene-forming)
Isolated from the plants Rosmarinus officinalis (rosemary) and Salvia miltiorrhiza. The enzyme from the plant Selaginella moellendorffii is mutifunctional and also catalyses EC 5.5.1.12, copalyl diphosphate synthase [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
the enzyme is involved in tanshinone biosynthesis
physiological function
Nicotiana benthamiana transient expression of copalyl diphosphate synthase CPS1 with isoform KSL1 results in the production of miltiradiene plus about 25% abietatriene; Nicotiana benthamiana transient expression of copalyl diphosphate synthase CPS1 with isoform KSL1 results in the production of miltiradiene plus about 25% abietatriene
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl diphosphate
miltiradiene + diphosphate
show the reaction diagram
labda-13-en-8-ol diphosphate
manoyl oxide + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
-
MDS is a bifunctional miltiradiene synthase catalyzing the cyclization reaction of geranylgeranyl diphosphate via (+)-copalyl diphosphate to afford miltiradiene as a final product
-
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
elevated expression in trichome of young leaf. Isoform KSL1 transcript level is less than 1% of expression of isoform KSL2; elevated expression in trichome of young leaf. Isoform KSL1 transcript level is less than 1% of expression of isoform KSL2
Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae
-
expression in Escherichia coli and Saccharomyces cerevisiae
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D391G/D392G
-
the mutant converts only (+)-copalyl diphosphate but not ent- or syn-copalyl diphosphate
D611G/D612G
-
the mutant converts geranylgeranyl diphosphate to ent-copalyl diphosphate
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
-
expression of copalyl diphosphate synthase and miltiradiene synthase in Saccharomyces cerevisiae, results in production of 4.2 mg/l miltiradiene. Miltiradiene production increases to 8.8 mg/l by improving supply of geranylgeranyl diphosphate by over-expression of a fusion gene of farnesyl diphosphate synthase ERG20 and endogenous geranylgeranyl diphosphate synthase BTS1 together with a heterologous geranylgeranyl diphosphate synthase from Sulfolobus acidocaldarius. Combinatorial overexpression of truncated 3-hydroxyl-3-methylglutaryl-CoA reductase with a mutated global regulatory factor upc2.1 and ERG20-BTS1-geranylgeranyl diphosphate synthase genes has synergetic effects on miltiradiene production, increasing titer to 61.8 mg/l. In fed-batch fermentation 488 mg/l miltiradiene can be produced