Information on EC 4.2.3.131 - miltiradiene synthase

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The expected taxonomic range for this enzyme is: Salvia miltiorrhiza

EC NUMBER
COMMENTARY
4.2.3.131
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RECOMMENDED NAME
GeneOntology No.
miltiradiene synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
(+)-copalyl diphosphate = miltiradiene + diphosphate
show the reaction diagram
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PATHWAY
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
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Diterpenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
(+)-copaly-diphosphate diphosphate-lyase (cyclizing, miltiradiene-forming)
Isolated from the plant Selaginella moellendorffii as a mutifunctional enzyme that also catalyses EC 5.5.1.12, copalyl diphosphate synthase [2]. In the plant Salvia miltiorrhiza the two enzymes are separate entities [1].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
kaurene synthase-like
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SmiKSL
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SmMDS
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ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
metabolism
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the enzyme is involved in tanshinone biosynthesis
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-copalyl diphosphate
miltiradiene + diphosphate
show the reaction diagram
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-
-
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?
(+)-copalyl diphosphate
miltiradiene + diphosphate
show the reaction diagram
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the enzyme is quite specific in its production of miltiradiene with more than 95% of the total product output
-
?
additional information
?
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MDS is a bifunctional miltiradiene synthase catalyzing the cyclization reaction of geranylgeranyl diphosphate via (+)-copalyl diphosphate to afford miltiradiene as a final product
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-
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Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Saccharomyces cerevisiae
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D391G/D392G
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the mutant converts only (+)-copalyl diphosphate but not ent- or syn-copalyl diphosphate
D611G/D612G
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the mutant converts geranylgeranyl diphosphate to ent-copalyl diphosphate