4.2.1.150: short-chain-enoyl-CoA hydratase
This is an abbreviated version!
For detailed information about short-chain-enoyl-CoA hydratase, go to the full flat file.
Reaction
Synonyms
3-hydroxyacyl-CoA dehydratase, 3-hydroxybutyryl-CoA dehydratase, CaCRT, CDYL, chromodomain Y-like protein, crotonase, crotonyl-CoA hydratase, crt, L-3-hydroxyacyl-CoA hydro-lyase, More, Mr-Crt, Mrub_2284, Msed_0399
ECTree
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Purification
Purification on EC 4.2.1.150 - short-chain-enoyl-CoA hydratase
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acetone precipitation, Sephadex G-200 gel filtration, and DEAE-Sephadex column chromatography
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recombinant C-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain B834 by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography and desalting gel filtration