Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Metallosphaera sedula |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
crotonyl-CoA | pH 8.0, 65°C | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, SDS-PAGE | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | Metallosphaera sedula | the enzyme is involved in autotrophic carbon fixation | (S)-3-hydroxybutanoyl-CoA | - |
? | |
crotonyl-CoA + H2O | Metallosphaera sedula DSM 5348 | the enzyme is involved in autotrophic carbon fixation | (S)-3-hydroxybutanoyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YDS4 | - |
- |
Metallosphaera sedula DSM 5348 | A4YDS4 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:autotrophically-grown cell | - |
Metallosphaera sedula | - |
culture condition:heterotrophically-grown cell | - |
Metallosphaera sedula | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 8 | pH 8.0, 65°C, native enzyme, autotrophically- or heterotrophically-grown cell | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
crotonyl-CoA + H2O | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula | (S)-3-hydroxybutanoyl-CoA | - |
? | |
crotonyl-CoA + H2O | bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula | (S)-3-hydroxybutanoyl-CoA | - |
? | |
crotonyl-CoA + H2O | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula DSM 5348 | (S)-3-hydroxybutanoyl-CoA | - |
? | |
crotonyl-CoA + H2O | bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula DSM 5348 | (S)-3-hydroxybutanoyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, SDS-PAGE | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
Msed_0399 | locus name, bifunctional crotonyl-CoA hydratase/(S)-3-hydroxybutanoyl-CoA dehydrogenase (EC 4.2.1.150/EC 1.1.1.35) | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
assay at | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Metallosphaera sedula |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in autotrophic carbon fixation | Metallosphaera sedula |