4.2.1.150: short-chain-enoyl-CoA hydratase

This is an abbreviated version!
For detailed information about short-chain-enoyl-CoA hydratase, go to the full flat file.

Reaction

Synonyms

3-hydroxyacyl-CoA dehydratase, 3-hydroxybutyryl-CoA dehydratase, CaCRT, CDYL, chromodomain Y-like protein, crotonase, crotonyl-CoA hydratase, crt, L-3-hydroxyacyl-CoA hydro-lyase, More, Mr-Crt, Mrub_2284, Msed_0399

ECTree

4 Lyases

4.2 Carbon-oxygen lyases

4.2.1 Hydro-lyases

4.2.1.150 short-chain-enoyl-CoA hydratase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
1124	AA Sequence
5	Cloned(Commentary)
1	Crystallization (Commentary)
6	Disease/ Diagnostics
22	General Information
1	General Stability
4	Inhibitors
2	kcat/KM [mM/s]
8	KM Value [mM]
3	Localization
8	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
18	Organism
17	Pathway
3	PDB ID
5	pH Optimum
1	pH Range
8	Protein Variants
6	Purification (Commentary)
3	Reaction
16	Reference
8	Source Tissue
2	Specific Activity [micromol/min/mg]
1	Storage Stability
25	Substrates and Products (Substrate)
12	Subunits
38	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
2	Turnover Number [1/s]

Cloned

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Cloned on EC 4.2.1.150 - short-chain-enoyl-CoA hydratase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Cloned (commentary) Search

expressed in Escherichia coli XL1-Blue cells

Clostridium acetobutylicum

286058

expression in Escherichia coli

Metallosphaera sedula

A4YDS4

727751

gene Mrub_2284, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain Rosetta (DE3), subcloning in Escherichia coli strain XL-1 Blue

Meiothermus ruber

D3PLE5

748405

recombinantly expressed in Escherichia coli

Metallosphaera sedula

A4YDS4

726748

the CaCRT coding gene (Met1-Arg261) is amplified by PCR using the chromosomal DNA of Clostridium acetobutylicum strain ATCC 824 as a template, recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain B834

Clostridium acetobutylicum

P52046

746965