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Literature summary for 4.2.1.150 extracted from
- Purification and characterization of cortonase from Clostridium acetobutylicum (1972), J. Biol. Chem., 247, 5266-5271.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization results in negligible loss of enzymatic activity | Clostridium acetobutylicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| crotonyl-CoA | - |
Clostridium acetobutylicum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
crotonyl-CoA | 25°C, pH 7.5 | Clostridium acetobutylicum | |
| 0.13 | - |
hexenoyl-CoA | 25°C, pH 7.5 | Clostridium acetobutylicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Clostridium acetobutylicum |
| 158000 | - |
sedimentation equilibrium studies | Clostridium acetobutylicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium acetobutylicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6155 | - |
25°C, pH 7.5 | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| a short-chain trans-2-enoyl-CoA + H2O | the enzyme is specific for short chain fatty acyl-CoA substrates and is sensitive to high concentrations of crotonyl-CoA. It requires a complete coenzyme A thioester substrate for efficient catalysis | Clostridium acetobutylicum | short-chain (3S)-3-hydroxyacyl-CoA | - |
? | |
| crotonyl-CoA + H2O | - |
Clostridium acetobutylicum | ? | - |
? | |
| hexenoyl-CoA + H2O | higher analogs are not hydrated | Clostridium acetobutylicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Clostridium acetobutylicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-hydroxybutyryl-CoA dehydratase | - |
Clostridium acetobutylicum |
| crotonase | - |
Clostridium acetobutylicum |
| crt | - |
Clostridium acetobutylicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Clostridium acetobutylicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Clostridium acetobutylicum |
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Release 2023.1 (January 2023)
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