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4.2.1.116: 3-hydroxypropionyl-CoA dehydratase

This is an abbreviated version!
For detailed information about 3-hydroxypropionyl-CoA dehydratase, go to the full flat file.

Word Map on EC 4.2.1.116

Reaction

3-hydroxypropanoyl-CoA
=
acryloyl-CoA
+
H2O

Synonyms

3-hydroxypropionyl-CoA dehydratase, 3-hydroxypropionyl-coenzyme A dehydratase, 3HPCD, HPCD, Ms3HPCD, Msed_2001

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.116 3-hydroxypropionyl-CoA dehydratase

Purification

Purification on EC 4.2.1.116 - 3-hydroxypropionyl-CoA dehydratase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3)-T1 by nickel affinity chromatography and gel filtration to homogeneity
recombinant His-tagged enzyme HPCD from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography