Information on EC 4.2.1.116 - 3-hydroxypropionyl-CoA dehydratase

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The expected taxonomic range for this enzyme is: Metallosphaera sedula

EC NUMBER
COMMENTARY hide
4.2.1.116
-
RECOMMENDED NAME
GeneOntology No.
3-hydroxypropionyl-CoA dehydratase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxypropanoyl-CoA = acryloyl-CoA + H2O
show the reaction diagram
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-hydroxypropanoate cycle
-
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3-hydroxypropanoate/4-hydroxybutanate cycle
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beta-alanine biosynthesis II
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Carbon fixation pathways in prokaryotes
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glyoxylate assimilation
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Microbial metabolism in diverse environments
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propanoyl-CoA degradation II
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propionyl-coA synthase
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CO2 fixation in Crenarchaeota
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxypropionyl-CoA hydro-lyase
Catalyses a step in the 3-hydroxypropanoate/4-hydroxybutanoate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [1]. The enzyme from Metallosphaera sedula acts nearly equally as well on (S)-3-hydroxybutanoyl-CoA but not (R)-3-hydroxybutanoyl-CoA [2].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-3-hydroxybutyryl-CoA
crotonyl-CoA + H2O
show the reaction diagram
no substrate: (R)-3-hydroxybutyryl-CoA
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-
?
3-hydroxypropanoyl-CoA
acryloyl-CoA + H2O
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.075
(S)-3-hydroxybutyryl-CoA
pH 8.6, 65°C
0.06
3-hydroxypropionyl-CoA
pH 8.6, 65°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
96
3-hydroxypropionyl-CoA
Metallosphaera sedula
A4YI89
pH 8.6, 65°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.4
extract of autotrophically grown cells, pH 8.6
3.1
extract of heterotrophically grown cells, pH 8.6
151
purified, recombinant protein, pH 8.6
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
half-maximal activity
9.5
half-maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23000
gel filtration
28300
1 * 29000, SDS-PAGE, 1 * 28300, calculated
29000
1 * 29000, SDS-PAGE, 1 * 28300, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
1 * 29000, SDS-PAGE, 1 * 28300, calculated