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4.2.1.116: 3-hydroxypropionyl-CoA dehydratase

This is an abbreviated version!
For detailed information about 3-hydroxypropionyl-CoA dehydratase, go to the full flat file.

Word Map on EC 4.2.1.116

Reaction

3-hydroxypropanoyl-CoA
=
acryloyl-CoA
 +
H2O

Synonyms

3-hydroxypropionyl-CoA dehydratase, 3-hydroxypropionyl-coenzyme A dehydratase, 3HPCD, HPCD, Ms3HPCD, Msed_2001

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.116 3-hydroxypropionyl-CoA dehydratase

Crystallization

Crystallization on EC 4.2.1.116 - 3-hydroxypropionyl-CoA dehydratase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified enzyme in complex with CoA, hanging drop vapor diffusion method, mixing of 0.001 ml of protein solution containing 41 mg/ml in 40 mM Tris-HCl, pH8.0, with 0.001 ml of reservoir solution, containing consisting of 10% w/v PEG 8000, 0.1 M sodium-potassium phosphate, pH 6.2, 0.2 M NaCl, and 10 mM EDTA, and equilibration against 0.05 ml of reservoir solution, 20°C, X-ray diffraction structure determination and analysis at 1.8 A resolution, modelling