Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.2.1.116: 3-hydroxypropionyl-CoA dehydratase

This is an abbreviated version!
For detailed information about 3-hydroxypropionyl-CoA dehydratase, go to the full flat file.

Word Map on EC 4.2.1.116

Reaction

3-hydroxypropanoyl-CoA
=
acryloyl-CoA
 +
H2O

Synonyms

3-hydroxypropionyl-CoA dehydratase, 3-hydroxypropionyl-coenzyme A dehydratase, 3HPCD, HPCD, Ms3HPCD, Msed_2001

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.116 3-hydroxypropionyl-CoA dehydratase

Engineering

Engineering on EC 4.2.1.116 - 3-hydroxypropionyl-CoA dehydratase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
production of the commercially promising platform chemical 3-hydroxypropionic acid (3-HP) via the propionyl-CoA pathway in genetically engineered Escherichia coli strain BL21(DE3). Propionate CoA-transferase from Megasphaera elsdenii and 3-hydroxypropionyl-CoA dehydratase (HPCD) from Chloroflexus aurantiacus are expressed along with propionyl-CoA dehydrogenase (PACD) from Candida rugosa, the 3-hydroxypropanoate titer of the resulting Escherichia coli Ec-PPH strain is improved by 6fold. When cultured at 30°C with 1% glucose in addition to propionate, 3-hydroxypropanoate production by Ec-PPH increases 2fold and 12fold compared to the cultivation at 37°C (4.23 mM) or without glucose (0.68 mM), respectively. Deletion of the ygfH gene encoding propionyl-CoA: succinate CoA-transferase from Ec-PPH (resulting in the strain Ec-DELTAY-PPH) leads to increase of 3-hydroxypropanoate production in shake flask experiments (15.04 mM), whereas the strain Ec-DELTAY-PPH with deletion of the prpC gene (encoding methylcitrate synthase in the methylcitrate cycle) produces 17.76 mM of 3-HP. The strain Ec-DELTAY-DELTAP-PPH with both ygfH and prpC genes deleted produces 24.14 mM of 3-HP, thus showing an 18fold increase in the 3-hydroxypropanoate titer in compare to the strain Ec-P. Disruption of the competing metabolic pathways. Established transgenic metabolic pathway, method, overview