4.2.1.11: phosphopyruvate hydratase

This is an abbreviated version!
For detailed information about phosphopyruvate hydratase, go to the full flat file.

Reaction

Synonyms

14-3-2 protein, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolase, 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glyceratehydrolyase, 2-phosphoglycerate dehydratase, 2-phosphoglycerate enolase, 2-phosphoglycerate hydrolase, 2-phosphoglycerate hydrolyase, 2-phosphoglyceric dehydratase, 2-phosphopyruvate hydrolyase, alpha,alpha-enolase, alpha-enolase, alpha-enolase 1, alpha-enolase 2, Alt a XI, beta,beta-enolase, beta-enolase, Cla h VI, Csenolase, cytotoxin B, EhENO, ENO, ENO-1, Eno-alpha, ENO-S, ENO1, Eno1p, Eno2, ENOA, EnoA I, EnoA1, ENOL, Enol-1, enolase, enolase 1, enolase 2, enolase alpha, enolase gamma, enolase S, enolase-1, enolase-alpha, enolase-beta, Enolase-phosphatase E1, enolase2p, Err2p, Err3p, gamma,gamma-enolase, gamma-enolase, hENO1, HLE1, human alpha-enolase, human neuron-specific enolase, hydratase, phosphoenolpyruvate, Laminin binding protein, Major allergen Alt a 11, MASA, MSE, Neural enolase, neuron specific enolase, neuron-specific enolase, neuronal enolase, NNE, Non-neural enolase, NSE, OSE1, p45, P46, Pfen, Pfeno, phosphoenolpyruvate enolase, phosphoenolpyruvate hydratase, Phosphopyruvate hydratase, plasminogen-binding alpha-enolase, R-NSE, r-Pfen, rBaEn, rSsEno, SEN, SjENO, Skeletal muscle enolase, SPM2, SsEno, SSO0913, streptococcal surface enolase, Tau-crystallin, tv-ENO1, tv-rENO1, XEP, yeast enolase

ECTree

     4 Lyases

         4.2 Carbon-oxygen lyases

             4.2.1 Hydro-lyases

                4.2.1.11 phosphopyruvate hydratase

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Results	in table
6	Activating Compound
62303	AA Sequence
35	Application
1	CAS Registry Number
77	Cloned(Commentary)
3	Cofactor
29	Crystallization (Commentary)
16005	Disease/ Diagnostics
16	Expression
101	General Information
2	General Stability
2	IC50 Value
110	Inhibitors
13	kcat/KM [mM/s]
24	Ki Value [mM]
119	KM Value [mM]
174	Localization
105	Metals/Ions
164	Molecular Weight [Da]
128	Natural Substrates/ Products (Substrates)
315	Organism
27	Pathway
274	PDB ID
62	pH Optimum
8	pH Range
1	pH Stability
16	pI Value
8	Posttranslational Modification
53	Protein Variants
119	Purification (Commentary)
4	Reaction
2	Reaction Type
271	Reference
1	Renatured (Commentary)
204	Source Tissue
101	Specific Activity [micromol/min/mg]
7	Storage Stability
329	Substrates and Products (Substrate)
133	Subunits
314	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
4	Temperature Range [°C]
13	Temperature Stability [°C]
13	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 4.2.1.11 - phosphopyruvate hydratase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 10 mM Tris-HCL, pH 7.5, stable

Clostridioides difficile

-

33627

-74°C, pH 7.0, stable for up to 10 months, provided that multiple freezing and thawing steps are avoided

Mus musculus

-

33625

4°C, 1 mg/ml or more, 0.1 M imidazole HCl, pH 7.0 and 2 mM MgCl2, stable for at least 2 months

Sus scrofa

-

33602

4°C, 15% ethylene glycol, stable for at least 3 months

Saccharomyces cerevisiae

-

33617

4°C, 70% saturated ammonium sulfate solution, stable for several months

Macaca mulatta

-

33601

4°C, stable for several months

Rattus norvegicus

-

33604

in the frozen state, stable for 6 months

Priestia megaterium

-

33609