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4.2.1.11: phosphopyruvate hydratase

This is an abbreviated version!
For detailed information about phosphopyruvate hydratase, go to the full flat file.

Word Map on EC 4.2.1.11

Reaction

2-phospho-D-glycerate
=
phosphoenolpyruvate
 +
H2O

Synonyms

14-3-2 protein, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolase, 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glyceratehydrolyase, 2-phosphoglycerate dehydratase, 2-phosphoglycerate enolase, 2-phosphoglycerate hydrolase, 2-phosphoglycerate hydrolyase, 2-phosphoglyceric dehydratase, 2-phosphopyruvate hydrolyase, alpha,alpha-enolase, alpha-enolase, alpha-enolase 1, alpha-enolase 2, Alt a XI, beta,beta-enolase, beta-enolase, Cla h VI, Csenolase, cytotoxin B, EhENO, ENO, ENO-1, Eno-alpha, ENO-S, ENO1, Eno1p, Eno2, ENOA, EnoA I, EnoA1, ENOL, Enol-1, enolase, enolase 1, enolase 2, enolase alpha, enolase gamma, enolase S, enolase-1, enolase-alpha, enolase-beta, Enolase-phosphatase E1, enolase2p, Err2p, Err3p, gamma,gamma-enolase, gamma-enolase, hENO1, HLE1, human alpha-enolase, human neuron-specific enolase, hydratase, phosphoenolpyruvate, Laminin binding protein, Major allergen Alt a 11, MASA, MSE, Neural enolase, neuron specific enolase, neuron-specific enolase, neuronal enolase, NNE, Non-neural enolase, NSE, OSE1, p45, P46, Pfen, Pfeno, phosphoenolpyruvate enolase, phosphoenolpyruvate hydratase, Phosphopyruvate hydratase, plasminogen-binding alpha-enolase, R-NSE, r-Pfen, rBaEn, rSsEno, SEN, SjENO, Skeletal muscle enolase, SPM2, SsEno, SSO0913, streptococcal surface enolase, Tau-crystallin, tv-ENO1, tv-rENO1, XEP, yeast enolase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.11 phosphopyruvate hydratase

Purification

Purification on EC 4.2.1.11 - phosphopyruvate hydratase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha,alpha-enolase and gamma,gamma-enolase
-
ammonium sulfate precipitation and gel filtration
ammonium sulfate precipitation, DEAE Sephadex A-50 gel filtration, Sephadex G-100 gel filtration
-
ammonium sulfate precipitation, DEAE-Sephadex A-50 gel filtration, CM-Sephadex gel filtration, and QAE-Sephadex gel filtration
-
beta,beta-enolase
-
by ion-exchange chromatography and gel filtration
DEAE and carboxymethyl columns, oligonucleotide affinity column
-
enolase 1, enolase II, enolase III
-
extracellular enolase identified from isolates of extracellular dextransucrase from cultures of Leuconostoc mesenteroides, accumulation as small crystals
for localization studies, immunochemistry and confocal microscopy
gamma,gamma-enolase
-
gel filtration
Plasmodium yoeliie XL17
-
gel filtration of recombinant protein
gel filtration, for in vitro import assays into mitochondria and three-hybrid analysis
-
gel filtration, for localization studies and tubulin-binding kinetics
gel filtration, recombinant enolase of Plasmodium falciparum for vaccination studies in mice
gelatin-agarose affinity column chromatography
glutathione-Sepharose 4B bead chromatography, gel filtration
-
glutathione-Sepharose beads chromatography, gel filtration
-
glutathione-Sepharose-4B chromatography
GSTrap FF column chromatography and Superdex 200 gel filtration
His-Bind column chromatography
His-Trap column chromatography
HiTrap Ni2+-chelating column chromatography and Superdex 75 gel filtration
ion exchange chromatography and gel filtration
isoforms S1, S2, S3
-
liver enzyme
-
MagneHis nickel-conjugated magnetic bead column chromatography, and gel filtration
-
muscle enzyme
-
muscle-stage larvae isolated by pepsin-HCl digestion from mice, recombinant enzyme by His Trap chelating column
-
native enzyme from muscle by ammonium sulfate fractionation and ion exchange chromatography
native enzyme partially by preparation of food vacuoles
-
Ni-nitrilotriacetic acid affinity chromatography, eluted with imidazole
-
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA column chromatography, gel filtration
Ni-NTA resin column chromatography
Ni-NTA Sepharose, ion-exchange chromatography, ammonium sulfate precipitation
-
Ni-NTA-agarose column chromatography, gel filtration
Ni2+-affinity column chromatography
-
Ni2+-charged HiTrap chelating column
nickel affinity column chromatography
nickel-affinity column chromatography and Superdex 200 gel filtration
partial
partial, using ammonium sulfate fractionation and gel filtration chromatography
preparative electrophoresis
-
purification of the wild-type and a mutant enzyme cloned in Escherichia coli using ammonium sulfate precipitation and Sephadex chromatography
Q-Sepharose column chromatography
-
recombinant GST-tagged ENO from Escherichia coli BL21 (DE3) by glutathione affinity chromatography, cleavage of the GST tag, and gel filtration
recombinant His-tagged enolase from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant protein
recombinant protein amounts are 10-15% of the total protein, purification from inclusion bodies yields 0.5 mg per liter, allergenic activity of the recombinant protein tested by ELIZA
recombinant protein and natural enolase
recombinant protein, for epitope mapping and identification of plasminogen-binding motifs
recombinant protein, gel filtration
recombinant protein, pET22/14-X vector, His-tag purification, SDS-PAGE
recombinant proteins, SDS-PAGE
recombinant soluble His-tagged enzyme from Escherichia coli by nickel affinity chromatography
to homogeneity, using ammonium sulfate precipitation and DEAE-Sephadex column chromatography
-
using affinity chromatography to isolate a his-tagged recombinant enzyme followed by cleavage of the tag
using affinity chromatography to isolate a his-tagged recombinant enzyme, purification from latex using ammonium sulfate precipitation and gel filtration chromatography
using anion- and cation exchange chromatography
using gel filtration chromatography
-
wild type and several mutant recombinant enzymes using chromatography on Sepharose
-