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4.2.1.11: phosphopyruvate hydratase

This is an abbreviated version!
For detailed information about phosphopyruvate hydratase, go to the full flat file.

Word Map on EC 4.2.1.11

Reaction

2-phospho-D-glycerate
=
phosphoenolpyruvate
 +
H2O

Synonyms

14-3-2 protein, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolase, 2-phospho-D-glycerate hydrolyase, 2-phospho-D-glyceratehydrolyase, 2-phosphoglycerate dehydratase, 2-phosphoglycerate enolase, 2-phosphoglycerate hydrolase, 2-phosphoglycerate hydrolyase, 2-phosphoglyceric dehydratase, 2-phosphopyruvate hydrolyase, alpha,alpha-enolase, alpha-enolase, alpha-enolase 1, alpha-enolase 2, Alt a XI, beta,beta-enolase, beta-enolase, Cla h VI, Csenolase, cytotoxin B, EhENO, ENO, ENO-1, Eno-alpha, ENO-S, ENO1, Eno1p, Eno2, ENOA, EnoA I, EnoA1, ENOL, Enol-1, enolase, enolase 1, enolase 2, enolase alpha, enolase gamma, enolase S, enolase-1, enolase-alpha, enolase-beta, Enolase-phosphatase E1, enolase2p, Err2p, Err3p, gamma,gamma-enolase, gamma-enolase, hENO1, HLE1, human alpha-enolase, human neuron-specific enolase, hydratase, phosphoenolpyruvate, Laminin binding protein, Major allergen Alt a 11, MASA, MSE, Neural enolase, neuron specific enolase, neuron-specific enolase, neuronal enolase, NNE, Non-neural enolase, NSE, OSE1, p45, P46, Pfen, Pfeno, phosphoenolpyruvate enolase, phosphoenolpyruvate hydratase, Phosphopyruvate hydratase, plasminogen-binding alpha-enolase, R-NSE, r-Pfen, rBaEn, rSsEno, SEN, SjENO, Skeletal muscle enolase, SPM2, SsEno, SSO0913, streptococcal surface enolase, Tau-crystallin, tv-ENO1, tv-rENO1, XEP, yeast enolase

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.11 phosphopyruvate hydratase

Specific Activity

Specific Activity on EC 4.2.1.11 - phosphopyruvate hydratase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.107
-
pH 7.5, 37°C, activity in undialyzed cell extracts
0.41
-
crude extract, at pH 7.8
0.79
-
substrate: 2-phospho-D-glycerate, 50°C, pH not specified in the publication, enzyme from starch-grown cells
0.9
-
crude extract, at pH 6.8
1.1
-
H159N mutant, pH 7.8, 21ºC
1.9
-
H159F mutant, pH 7.8, 21ºC
10.3
-
-
10050
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Zn2+
11.29
substrate phosphoenolpyruvate, 22°C, pH not specified in the publication
111
room temperature, pH 7.5, MgCl2, KCl, 2-phosphoglyceric acid
1118
-
dimeric enzyme form
120
-
muscle enolase
124
pH 7.5, 22ºC
13.8
pH 8, 70°C
15.87
purified recombinant enzyme, substrate phosphoenolpyruvate, pH 7.4, 20°C
197.4
-
plastidic isoenzyme
22
pH 7.4, 25ºC
23.2
-
SPM2, 0.25% Triton X-100
248
-
N207A mutant, pH 7.8, 21ºC
252.4
-
cytosolic isoenzyme
260
-
pH 8.1, room temperature
3.3
-
H159A mutant, pH 7.8, 21ºC
30
-
2-phospho-D-glycerate, pH 7.4, 20°C, Tris/HCl
30.71
substrate 2-phospho-D-glycerate, 22°C, pH not specified in the publication
31.2
-
after 76.1fold purification, at pH 7.8
35
-
pH 6.8, 30ºC
35.81
purified recombinant enzyme, substrate 2-phospho-D-glycerate, pH 7.4, 20°C
3909
purifed recombinant enzyme, pH 8.5, 37°C, no metal ion added
442
-
wild type, pH 7.8, 21ºC
60 - 70
60.72
pH not specified in the publication, temperature not specified in the publication
64.6
-
liver enolase
67
recombinant wild-type enzyme, pH 7.1, 25ºC
67.4
-
enolase from synaptosomal cytoplasm
68
-
enolase II
69.6
-
-
700
-
pH 7.6, temperature not specified in the publication
75
-
after 83fold purification, at pH 6.8
76.2
-
-
77
recombinant E414L mutant, pH 7.1, 25ºC
79.8
-
brain enolase III
82.2
-
-
82.8
-
brain enolase I
85
pH 7.6, 25ºC
86.54
-
-
9091
purifed recombinant enzyme, pH 8.5, 37°C, 10 mM Mg2+
91
-
muscle enolase
918
-
monomeric enzyme form
92.3
-
muscle enolase
additional information