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4.1.99.2: tyrosine phenol-lyase

This is an abbreviated version!
For detailed information about tyrosine phenol-lyase, go to the full flat file.

Word Map on EC 4.1.99.2

Reaction

L-tyrosine
+
H2O
=
phenol
 +
pyruvate
 +
NH3

Synonyms

beta-tyrosinase, Fn-TPL, L-tyrosine phenol-lyase, phenol-lyase, tyrosine, TnaA, TPL, tyrosine phenol lyase, tyrosine phenol-lyase, tyrosine-phenol lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.2 tyrosine phenol-lyase

Temperature Stability

Temperature Stability on EC 4.1.99.2 - tyrosine phenol-lyase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 80
-
at 18°C the enzyme activity is about 20% of the maximal activity, when heated for 20 min, the wild type enzyme remains stable up to 55°C in a 0.1 M potassium phosphate buffer (pH 8.0), the half-inactivation temperature is calculated to be 62.2°C
35
-
purified native enzyme, half-life is 130 min
45
-
purified native enzyme, half-life is 52 min
55
-
purified native enzyme, half-life is 10 min
60
Aeromonas phenologenes
-
10 min, about 45% loss of activity
85
-
20 min, about 25% loss of activity
90
-
20 min, about 90% loss of activity