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Literature summary for 4.1.99.2 extracted from
- Purification and characterization of tyrosine phenol lyase from Citrobacter freundii (2013), Appl. Biochem. Biotechnol., 171, 2040-2052.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | tyrosine phenol lyase is an important enzyme for the synthesis of pharmaceutical aromatic amino acids, viz. L-tyrosine and L-DOPA | Citrobacter freundii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.446 | - |
L-tyrosine | pH 8.5, 30°C | Citrobacter freundii |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Citrobacter freundii | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
4 * 52000, SDS-PAGE | Citrobacter freundii |
| 220000 | - |
native enzyme, native PAGE | Citrobacter freundii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-3-4 dihydroxyphenylalanine + H2O | Citrobacter freundii | L-DOPA, alpha-beta elimination | catechol + pyruvate + NH3 | - |
r | |
| L-tyrosine + H2O | Citrobacter freundii | alpha-beta elimination | phenol + pyruvate + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 35fold to homogeneity by ammonium sulfate fractionation, dialysis, and hydrophobic interaction chromatography | Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.1 | - |
purified enzyme, pH 8.5, 30°C | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-3-4 dihydroxyphenylalanine + H2O | L-DOPA, alpha-beta elimination | Citrobacter freundii | catechol + pyruvate + NH3 | - |
r | |
| L-asparagine + H2O | - |
Citrobacter freundii | ? | - |
? | |
| L-methionine + H2O | - |
Citrobacter freundii | ? | - |
? | |
| L-phenylalanine + H2O | low activity | Citrobacter freundii | ? | - |
? | |
| L-tyrosine + H2O | alpha-beta elimination | Citrobacter freundii | phenol + pyruvate + NH3 | - |
r | |
| L-valine + H2O | - |
Citrobacter freundii | ? | - |
? | |
| additional information | alanine, arginine, aspartate, cysteine, glutamine, glutamate, glycine, histidine, isoleucine, leucine, lysine, proline, serine, threonine, tryptophan are poor or no substrates, substrate specificity of purified tyrosine phenol lyase, overview | Citrobacter freundii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | N-terminal sequences comparisons, overview | Citrobacter freundii |
| tetramer | 4 * 52000, SDS-PAGE | Citrobacter freundii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine phenol lyase | - |
Citrobacter freundii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Citrobacter freundii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | activity range, profile, overview | Citrobacter freundii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
purified native enzyme, half-life is 130 min | Citrobacter freundii |
| 45 | - |
purified native enzyme, half-life is 52 min | Citrobacter freundii |
| 55 | - |
purified native enzyme, half-life is 10 min | Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Citrobacter freundii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on, highest activity at 0.015-0.02 mM | Citrobacter freundii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | tyrosine phenol lyase catalyses the biosynthesis of L-tyrosine/L-DOPA from ammonium, pyruvate and phenolic substrates through the reverse of alpha- and beta-elimination reaction | Citrobacter freundii |
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Release 2023.1 (January 2023)
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