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4.1.99.2: tyrosine phenol-lyase

This is an abbreviated version!
For detailed information about tyrosine phenol-lyase, go to the full flat file.

Word Map on EC 4.1.99.2

Reaction

L-tyrosine
+
H2O
=
phenol
+
pyruvate
+
NH3

Synonyms

beta-tyrosinase, Fn-TPL, L-tyrosine phenol-lyase, phenol-lyase, tyrosine, TnaA, TPL, tyrosine phenol lyase, tyrosine phenol-lyase, tyrosine-phenol lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.2 tyrosine phenol-lyase

Purification

Purification on EC 4.1.99.2 - tyrosine phenol-lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
-
mutant enzyme A13V
-
mutant enzyme His343Ala
-
native enzyme 35fold to homogeneity by ammonium sulfate fractionation, dialysis, and hydrophobic interaction chromatography
-
partial
partial purification by methanol treatment
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant enzyme from Escherichia coli strain SVS370/pTZTPL
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration
Resource Q ion exchange chromatography and Phenyl Superose Phenyl Superose column chromatography
-
wild-type protein and mutants T451A/A13V/E83K and T129I/A13V purified to homogeneity with purification yields of over 40%
-