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4.1.99.2: tyrosine phenol-lyase

This is an abbreviated version!
For detailed information about tyrosine phenol-lyase, go to the full flat file.

Word Map on EC 4.1.99.2

Reaction

L-tyrosine
+
H2O
=
phenol
+
pyruvate
+
NH3

Synonyms

beta-tyrosinase, Fn-TPL, L-tyrosine phenol-lyase, phenol-lyase, tyrosine, TnaA, TPL, tyrosine phenol lyase, tyrosine phenol-lyase, tyrosine-phenol lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.2 tyrosine phenol-lyase

Cloned

Cloned on EC 4.1.99.2 - tyrosine phenol-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expressed in Escherichia coli strain BL21
-
expressed in Escherichia coli SVS370 cells
-
expressed in Escherichia coli XL-1 Blue cells
-
expression in Escherichia coli
expression in Escherichia coli, fusion to the lac and tac promoters, erpressed at high levels in the presence of isopropyl-beta-D-thiogalactopyranoside or lactose as inducer
-
gene tnaA, recombinant expression in Escherichia coli strain BL21(DE3)
gene tnaA, recombinant expression of codon-optimized His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene tnaA, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10beta
gene tnaA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene tpl, expression of wild-type and mutant enzymes in Escherichia coli strain SVS 370
-
gene tpl, recombinant expression in Escherichia coli strain SVS370/pTZTPL
plasmid pHCE IIB-TPL ligated at 4°C for 24 h with a T4 DNA ligase, products electrotransformed into Escherichia coli JM83
-
Targeted gene disruptions are performed. Gene replacement vectors for the hpd, pobA, phhA, and aroP1 genes are created from pJQ200SK with different primers. Vectors are used to mutate the selected genes in Pseudomonas putida S12TPL3c by homologous recombination.
-
the plasmid pTZTPL is constructed for expression of the protein in Escherichia coli SVS370 cells