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4.1.99.14: spore photoproduct lyase

This is an abbreviated version!
For detailed information about spore photoproduct lyase, go to the full flat file.

Word Map on EC 4.1.99.14

Reaction

(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA)
=
thymidylyl-(3'->5')-thymidylate (in double-helical DNA)

Synonyms

Bs SP lyase, Bs SPL, Ca SP lyase, Gt SP lyase, Gt SPL, GTNG_2348, More, SP lyase, SPL, SPL(Ca), spl-1, SplB, SplG, spore photoproduct lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.14 spore photoproduct lyase

Purification

Purification on EC 4.1.99.14 - spore photoproduct lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged enzyme from Escherichia coli strain Tuner (DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli Tuner(DE3)pLysS by nickel affinity chromatography to homogeneity
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and heparin affinity chromatography. The iron-sulfur cluster of the purified proteins is then reconstituted
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis to over 95% purity
recombinant His6-tagged enzyme from Escherichia coli strain pLysS (DE3) by nickel affinity chromatography and gel filtration
-
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and cation exchange chromatography
-
recombinant N- or C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant N-terminal His6-tagged enzyme from Escherichia coli strain Tuner(DE3)pLysS by nickel affinity chromatography, anaerobic dialysis, and ultrafiltration
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Tuner(DE3)-pLysS by nickel affinity chromatography, dialysis under anaerobic conditions, and ultrafiltration
recombinant wild-type, selenomethionine-labeled, and mutant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel and heparin affinity chromatography
using a HisTrap HP 5-mL column
-
using Ni-NTA chromatography