Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.1.99.14: spore photoproduct lyase

This is an abbreviated version!
For detailed information about spore photoproduct lyase, go to the full flat file.

Word Map on EC 4.1.99.14

Reaction

(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA)
=
thymidylyl-(3'->5')-thymidylate (in double-helical DNA)

Synonyms

Bs SP lyase, Bs SPL, Ca SP lyase, Gt SP lyase, Gt SPL, GTNG_2348, More, SP lyase, SPL, SPL(Ca), spl-1, SplB, SplG, spore photoproduct lyase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.99 Other carbon-carbon lyases
                4.1.99.14 spore photoproduct lyase

Cloned

Cloned on EC 4.1.99.14 - spore photoproduct lyase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli as a His-tagged fusion protein
functional expression of N-terminally His6-tagged enzyme in Escherichia coli strain pLysS (DE3)
-
gene GTNG_2348, expression of His6-tagged native and selenomethionine-labeled wild-type enzyme and His6-tagged mutant enzymes in Escherichia coli strain BL21(DE3)
gene GTNG_2348, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene spl-1 or splB, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene splB, functional expression of His6-tagged enzyme in Escherichia coli Tuner(DE3)pLysS
-
gene splB, overexpression of His6-tagged wild-type and mutant enzymes and co-expression with plasmid pDB1282, in Escherichia coli strain BL21(DE3)
-
gene splB, overexpression of N- or C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene splB, overexpression of N-terminally His-tagged enzyme in Escherichia coli strain Tuner (DE3)
-
gene splB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) from plasmid pDB 1282 that confers ampicillin resistance and harbors an Escherichia coli operon that is involved in the biosynthesis of FeS clusters, it may facilitate incorporation of the FeS clusters into the apoprotein of SPL
gene splB, recombinant expression of N-terminal His6-tagged enzyme in Escherichia coli strain Tuner(DE3)pLysS
gene splB, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Tuner(DE3)-pLysS
heterologously expressed in Escherichia coli as a His-tagged fusion protein
-
overexpression in Escherichia coli
-