Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | A0A0E0TDM3 | DSM 22 | - |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone is reported. Repair studies show that the 5'-3' (5R) analogue, incorporated in oligonucleotides, is efficiently repaired | Geobacillus stearothermophilus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SplG | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Geobacillus stearothermophilus |
37 | - |
assay at | Geobacillus stearothermophilus |