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4.1.3.30: Methylisocitrate lyase

This is an abbreviated version!
For detailed information about Methylisocitrate lyase, go to the full flat file.

Word Map on EC 4.1.3.30

Reaction

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate
=
succinate
+
pyruvate

Synonyms

2-Methylisocitrate lyase, Lyase, methylisocitrate, MCL, Mcl1, methylisocitrate lyase, MICL, mmgF, PrpB, TgPrpB, yqiQ

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.30 Methylisocitrate lyase

Cloned

Cloned on EC 4.1.3.30 - Methylisocitrate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
fusion protein with green fluorescent protein
-
gene mcl, phylogenetic analysis
-
gene prpB, phylogenetic analysis, C-terminally Ty-tagged version of TgPrpB is located in the cytosolic fraction whereas an N-terminally myc-tagged TgPrpB is partitioned in both the cytosol and organellar fractions, mycPrpB is produced as a precursor of about 40 kDa that corresponds to the predicted size of full-length PrpB and this form is detectable with anti-myc
His-tagged version expressed in Escherichia coli BL21(DE3)Rosetta2
-