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Literature summary for 4.1.3.30 extracted from

  • Grimek, T.L.; Holden, H.; Rayment, I.; Escalante-Semerena, J.C.
    Residues C123 and D58 of the 2-methylisocitrate lyase (PrpB) enzyme of Salmonella enterica are essential for catalysis (2003), J. Bacteriol., 185, 4837-4843.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.3.30

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 5 mM, required for optimal activity Salmonella enterica
glutathione required for optimal activity Salmonella enterica

Cloned(Commentary)

Cloned (Comment) Organism
-
 Salmonella enterica

Protein Variants

Protein Variants Comment Organism
C123A no enzyme activity Salmonella enterica
D58A no enzyme activity Salmonella enterica
H125A decreased kcat Salmonella enterica
K121A decreased kcat Salmonella enterica
R122K decreased kcat Salmonella enterica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, H125A mutant Salmonella enterica
19
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, wild-type Salmonella enterica
22
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, R122K mutant Salmonella enterica
57
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, K121A mutant Salmonella enterica

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Salmonella enterica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 4 * 32000, SDS-PAGE Salmonella enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Salmonella enterica enzyme in methylcitrate cycle ?
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
-
 serovar typhimurium LT2
-

Reaction

Reaction Comment Organism Reaction ID
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = succinate + pyruvate mechanism Salmonella enterica
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate syn: methylisocitrate Salmonella enterica Pyruvate + succinate
-
 ?
additional information no substrate: isocitrate Salmonella enterica ?
-
 ?
additional information enzyme in methylcitrate cycle Salmonella enterica ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Salmonella enterica

Synonyms

Synonyms Comment Organism
PrpB
-
 Salmonella enterica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Salmonella enterica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 14% activity after 10 min Salmonella enterica
60
-
 no detectable activity Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, K121A mutant Salmonella enterica
0.14
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, H125A mutant Salmonella enterica
38
-
 Mg2+ pH 7.5, 37°C Salmonella enterica
55
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, R122K mutant Salmonella enterica
105
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7.5, 37°C, wild-type Salmonella enterica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Salmonella enterica

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 16% activity at pH 6, 96% activity at pH 8.5 Salmonella enterica