Cloned (Comment) | Organism |
---|---|
gene prpB, phylogenetic analysis, C-terminally Ty-tagged version of TgPrpB is located in the cytosolic fraction whereas an N-terminally myc-tagged TgPrpB is partitioned in both the cytosol and organellar fractions, mycPrpB is produced as a precursor of about 40 kDa that corresponds to the predicted size of full-length PrpB and this form is detectable with anti-myc | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | TgPrpB gene disrupture by double homologous gene replacement in RH tachyzoites. The enzyme-deficient prpb-ko mutant is complemented by expression of wild-type myc-tagged TgPrpB | Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | exclusively, subcellular localization analysis | Toxoplasma gondii | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, about, recombinant N-terminally myc-tagged pro-PrpB, SDS-PAGE | Toxoplasma gondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | Q2L8W6 | gene prpB | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | recombinant N-terminally myc-tagged PrpB is produced as a precursor of about 40 kDa | Toxoplasma gondii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, about, recombinant N-terminally myc-tagged pro-PrpB, SDS-PAGE | Toxoplasma gondii |
Synonyms | Comment | Organism |
---|---|---|
2-Methylisocitrate lyase | - |
Toxoplasma gondii |
TgPrpB | - |
Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
evolution | the 2-methylcitrate cycle enzymes have been acquired via horizontal gene transfer, PrpB phylogenetic analysis, overview | Toxoplasma gondii |
malfunction | an enzyme-disruption mutant prpb-ko shows accumulation of 2-methylcitrate cycle intermediate metabolites, enzyme substrates accumulate in parasites deficient in the enzyme and its absence confers increased sensitivity to propionic acid | Toxoplasma gondii |
physiological function | the enzyme is involved in the 2-methylcitrate cycle required to metabolize and detoxify propionate by oxidation to pyruvate, but the rate-limiting enzyme 2-methylisocitrate lyase is dispensable for parasite survival | Toxoplasma gondii |