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4.1.1.37: uroporphyrinogen decarboxylase

This is an abbreviated version!
For detailed information about uroporphyrinogen decarboxylase, go to the full flat file.

Word Map on EC 4.1.1.37

Reaction

uroporphyrinogen III
=
Coproporphyrinogen III
 + 4 CO2

Synonyms

ch-UroD, Decarboxylase, uroporphyrinogen, Hem12p, PCL, Porphyrinogen carboxy-lyase, rl-UroD, tobacco UROD, UORO-D, UPD, URO-D, Uro-decarboxylase, uro-III decarboxylase, UroD, UROD protein, UROD1, uroporphyrinogen decarboxylase, uroporphyrinogen decarboxylase 1, Uroporphyrinogen III decarboxylase, uroporphyrinogen-decarboxylase, uroporphyrinogen-III decarboxylase

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.1 Carboxy-lyases
                4.1.1.37 uroporphyrinogen decarboxylase

Purification

Purification on EC 4.1.1.37 - uroporphyrinogen decarboxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
IMAC column chromatography, Mono Q column chromatography, and Superdex 70 gel filtration
-
isoenzymes I and II
-
mutant URO-D proteins G156D, F232L, and I260T
Ni-nitriloacetic acid column chromatography
-
Ni-NTA column
Ni-NTA column chromatography
Ni-NTA column chromatography and gel filtration
partial, Superdex 75 gel filtration
-
substrate ligand column is developed by covalently linking coproporphyrin-III to a Sepharose resin. The ligand-resin chromatography step rapidly separates coproporphyrinogen oxidase from uroporphyrinogen decarboxylase
-
wild-type and mutant enzymes
-