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3.5.3.4: allantoicase

This is an abbreviated version!
For detailed information about allantoicase, go to the full flat file.

Word Map on EC 3.5.3.4

Reaction

Allantoate
+
H2O
=
(S)-ureidoglycolate
+
Urea

Synonyms

AaALLC, AAH, Allantoate amidinohydrolase, allantoate amidohydrolase, allantoicase, Allantoine amidinohydrolase, ALLC, XAlc

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.3 In linear amidines
                3.5.3.4 allantoicase

Purification

Purification on EC 3.5.3.4 - allantoicase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gravity flow metal chelate column chromatography
-
in the presence of Mn2+ ions and chromatography on Ecteola cellulose at 72°C
-
nickel-nitrilotriacetic acid column chromatography and gel filtration on a Superdex 75 column equilibrated in Tris-HCl, pH 7.5, 200 mM NaCl, 10 mM 2-mercaptoethanol
-
recombinant enzyme