Information on EC 3.5.3.4 - allantoicase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.5.3.4
-
RECOMMENDED NAME
GeneOntology No.
allantoicase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
allantoate + H2O = (S)-ureidoglycolate + urea
show the reaction diagram
-
-
-
-
allantoate + H2O = (S)-ureidoglycolate + urea
show the reaction diagram
thermodynamic data
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of linear amidines
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
allantoin degradation to ureidoglycolate I (urea producing)
-
-
Purine metabolism
-
-
Metabolic pathways
-
-
Microbial metabolism in diverse environments
-
-
SYSTEMATIC NAME
IUBMB Comments
allantoate amidinohydrolase
Also hydrolyses (R)-ureidoglycolate to glyoxylate and urea.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
AaALLC
Aedes aegypti NH-Rockefeller
-
-
Allantoate amidinohydrolase
-
-
-
-
Allantoate amidinohydrolase
-
-
Allantoate amidinohydrolase
-
-
allantoicase
-
allantoicase
Aedes aegypti NH-Rockefeller
-
-
allantoicase
-
-
allantoicase
-
-
allantoicase
-
YIR029W
allantoicase
-
-
Allantoine amidinohydrolase
-
-
-
-
ALLC
Aedes aegypti NH-Rockefeller
-
-
CAS REGISTRY NUMBER
COMMENTARY
9025-21-2
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
female mosquitoes; NH-Rockefeller strain
SwissProt
Manually annotated by BRENDA team
Aedes aegypti NH-Rockefeller
NH-Rockefeller strain
SwissProt
Manually annotated by BRENDA team
Mantis sp.
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Penicillium citreo-viride
-
-
-
Manually annotated by BRENDA team
prawn
-
-
-
Manually annotated by BRENDA team
enzyme also has allantoinase activity, EC 3.5.2.5
-
-
Manually annotated by BRENDA team
; strain S288c
-
-
Manually annotated by BRENDA team
mackerel
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
Penicillium citreo-viride
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
prawn, Mantis sp.
-
-
-
?
(-)-ureidoglycolate
glyoxylate + urea
show the reaction diagram
Mycobacterium scrofulaceum W200
-
-
-
?
(-)-ureidoglycolate + urea
allantoate + H2O
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
?
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
?
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
Penicillium citreo-viride
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
prawn, Mantis sp.
-
-
-
r
allantoate + H2O
(-)-ureidoglycolate + urea
show the reaction diagram
Mycobacterium scrofulaceum W200
-
-
-
r
allantoate + H2O
(S)-ureidoglycolate + urea
show the reaction diagram
-
-
?
allantoate + H2O
(S)-ureidoglycolate + urea
show the reaction diagram
-
-
?
allantoate + H2O
(S)-ureidoglycolate + urea
show the reaction diagram
alternate metabolic pathway for urea synthesis, via an amphibian-like uricolytic pathway
-
?
allantoate + H2O
(S)-ureidoglycolate + urea
show the reaction diagram
Aedes aegypti NH-Rockefeller
alternate metabolic pathway for urea synthesis, via an amphibian-like uricolytic pathway
-
?
allantoate + H2O
?
show the reaction diagram
-
-
-
-
-
allantoate + H2O
ureidoglycolate + urea
show the reaction diagram
-
-
-
?
allantoate + H2O
ureidoglycolate + urea
show the reaction diagram
-
-
?
allantoic acid + H2O
ureidoglycolate + urea
show the reaction diagram
-
-
-
?
allantoic acid + H2O
ureidoglycolate + urea
show the reaction diagram
alternate metabolic pathway for urea synthesis, via an amphibian-like uricolytic pathway
-
?
allantoic acid + H2O
ureidoglycolate + urea
show the reaction diagram
Aedes aegypti NH-Rockefeller
alternate metabolic pathway for urea synthesis, via an amphibian-like uricolytic pathway
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
allantoate + H2O
(S)-ureidoglycolate + urea
show the reaction diagram
A9XTM8
-
-
?
allantoate + H2O
?
show the reaction diagram
-
-
-
-
-
allantoate + H2O
(S)-ureidoglycolate + urea
show the reaction diagram
Aedes aegypti NH-Rockefeller
A9XTM8
-
-
?
allantoic acid + H2O
ureidoglycolate + urea
show the reaction diagram
Aedes aegypti, Aedes aegypti NH-Rockefeller
A9XTM8
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Bivalent cations
-
-
Mn2+
-
restores activity after treatment with EDTA
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(-)-ureidoglycolate
-
-
Allantoate
-
with (-)-ureidoglycolate as substrate
Ca2+
1 mM, about 50% of inhibition, reversed by EDTA
diethyldicarbonate
-
-
glycolic acid
-
-
glycolic acid
-
-
Hydantoic acid
-
-
iodoacetate
-
slight
N-Acetylimidazole
-
-
N-Carbamoyl-(R)-asparagine
-
-
p-hydroxymercuribenzoate
-
-
Mg2+
1 mM, about 50% of inhibition, reversed by EGTA
additional information
not inhibitory: Ca2+, Mg2+, EDTA, EGTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
EDTA
1 mM, more than 200% of initial activity
EGTA
1 mM, about 200% of initial activity
additional information
no activation by EDTA or EGTA
-
additional information
increased expression after blood meal at the end of digestion
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.7
(-)-ureidoglycolate
-
-
4.5
Allantoate
pH 7.0, 25C
8.44
Allantoate
; from kidney
250
Allantoate
pH 7.0, 25C
additional information
additional information
-
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
750
Allantoate
Chlamydomonas reinhardtii
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.9
-
30C, pH 7.9
additional information
-
-
additional information
-
-
additional information
-
-
additional information
labelled uric acid metabolized to urea and excreted as nitrogenous waste through an uricolytic pathway, effect of intermediate products of the uricolytic pathway on urea synthesis analyzed by feeding mosquitoes with unlabeled intermediates, induced gene expression of allantoicase in response to blood meal feeding argues for a functional role of the uricolytic pathway in urea synthesis, significant increase in uric acid levels in whole-body extracts, reduction in labelled urea excretion
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
; gene expression in, regulated after blood meal
Manually annotated by BRENDA team
Aedes aegypti NH-Rockefeller
-
; gene expression in, regulated after blood meal
-
Manually annotated by BRENDA team
feeding experiments, collection of feces and analysis by mass spectrometry
Manually annotated by BRENDA team
Aedes aegypti NH-Rockefeller
-
feeding experiments, collection of feces and analysis by mass spectrometry
-
Manually annotated by BRENDA team
Mantis sp.
-
-
Manually annotated by BRENDA team
; gene expression in, regulated after blood meal
Manually annotated by BRENDA team
Aedes aegypti NH-Rockefeller
-
-
-
Manually annotated by BRENDA team
kinetics of incorporation of labelled nitrogen
Manually annotated by BRENDA team
Aedes aegypti NH-Rockefeller
-
kinetics of incorporation of labelled nitrogen
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Mantis sp., prawn, Scomber japonicus
-
membrane
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
11000
-
sedimentation diffusion equilibrium, 0.9-S-allantoicase
172139
20000
-
SDS-PAGE
660730
38580
-
-
666868
43200
-
calculated from sequence of cDNA
664657
154000
-
sedimentation, 10.8-S-allantoicase
172139
200000
-
gel filtration
172151
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
x * 47200, deduced from gene sequence, x * 46500, SDS-PAGE
?
x * 44700, deduced from gene sequence, x * 43700, SDS-PAGE
dimer
-
analytical gel filtration
hexamer
-
6 * 34000, SDS-PAGE after 2-mercaptoethanol treatment
hexamer
-
dimer of trimers, crystallization data
monomer
-
X-ray crystallography using the multi-wavelength anomalous dispersion method
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
enzyme contains glucosamine
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; Se-Met-labeled protein crystals grown from a mixture in a 1:1.5 ratio of 8 mg/ml protein solution and a reservoir solution of 7% PEG8000, 12% PEG400, and 100 mM HEPES, pH 7.5
-
nanodroplet vapor diffusion method
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 10
-
-
172151
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
40
15 min, loss of about 50% of activity
172150
50
-
34% loss of activity after 30 min, 79% loss of activity after 90 min
172143
75
-
in presence of Mn2+ stable up to 75C, in absence of Mn2+ completely inactivated within 5 min
34475
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing and thawing, stable
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 50 mM phosphate buffer, pH 7.0, stable
4C, above, rapid loss of activity
-15C, 0.02 M Tris-HCl buffer, pH 7.5, 6 months,
-
-20C, 50 mM phosphate buffer, pH 7.0, stable
4C, above, rapid loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
; in the presence of Mn2+ ions and chromatography on Ecteola cellulose at 72C
-
gravity flow metal chelate column chromatography
-
nickel-nitrilotriacetic acid column chromatography and gel filtration on a Superdex 75 column equilibrated in Tris-HCl, pH 7.5, 200 mM NaCl, 10 mM 2-mercaptoethanol
-
; recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expressed in Escherichia coli
-
expressed in Escherichia coli
-
expressed in Escherichia coli strain DL41
-
expression in Escherichia coli
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
inhibition by divalent cations is reversed by excess of chaletors of these cations
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pharmacology
possibility to develop metabolism-based strategies for mosquito control
pharmacology
Aedes aegypti NH-Rockefeller
-
possibility to develop metabolism-based strategies for mosquito control
-