3.4.24.70: oligopeptidase A

This is an abbreviated version!
For detailed information about oligopeptidase A, go to the full flat file.

Word Map on EC 3.4.24.70

3.4.24.70

prolyl

typhimurium

dipeptidyl

carboxypeptidase

neuropeptides

endopeptidases

oxyanion

subtilisin

octapeptide

post-proline

thimet

neurolysin

z-pro-prolinal

bell-shaped

product-like

scissile

3.4.24.15

arrhenius

metallopeptidase

bradykinin

Reaction

hydrolysis of oligopeptides, with broad specificity. Gly or Ala commonly occur as P1 or P1' residues, but additional information distant residues are also important, as is shown by the fact that Z-Gly-Pro-Gly-/-Gly-Pro-Ala is cleaved, but not Z-(Gly)5 =

Synonyms

68000-M Signalpeptide hydrolase, More, oligopeptidase A, OpdA, prlC, TOP, Top1, Top2

ECTree

3 Hydrolases

3.4 Acting on peptide bonds (peptidases)

3.4.24 Metalloendopeptidases

3.4.24.70 oligopeptidase A

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Results	in table
13052	AA Sequence
2	CAS Registry Number
14	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
1	Expression
7	General Information
23	Inhibitors
14	kcat/KM [mM/s]
15	KM Value [mM]
6	Localization
18	Metals/Ions
4	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
62	Organism
1	PDB ID
3	pH Optimum
1	pH Range
3	Protein Variants
10	Purification (Commentary)
2	Reaction
1	Reaction Type
60	Reference
1	Source Tissue
5	Specific Activity [micromol/min/mg]
1	Storage Stability
57	Substrates and Products (Substrate)
3	Subunits
57	Synonyms
6	Temperature Optimum [°C]
14	Turnover Number [1/s]

Cloned

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Cloned on EC 3.4.24.70 - oligopeptidase A

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Escherichia coli, prlC-gene

2 entries

expressed from pHis3-OpdA

Escherichia coli

667317

expression of His-tagged wild-type OpdA and mutants in Escherichia coli strain DH5alpha

Escherichia coli

707285

gene opdA, DNA and amino acid sequence determination and anaylsis, expression of the His-tagged enzyme in strain BL21(DE3)

Escherichia coli K-12

683121

gene prlC, recombinant expression of N-terminally His-tagged enzyme in Escherichia ocli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha

Leptospira interrogans serovar Copenhageni

Q72SL8

754699

N-terminally His-tagged isozyme overexpression in Escherichia coli BL21 Rosetta (DE3)

nucleotide sequences

opdA-gene

Salmonella typhimurium

2 entries